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首页> 外文期刊>Medical Sciences >The 12-HHT/BLT2/NO Axis Is Associated to the Wound Healing and Skin Condition in Different Glycaemic States
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The 12-HHT/BLT2/NO Axis Is Associated to the Wound Healing and Skin Condition in Different Glycaemic States

机译：12-HHT / BLT2 / NO轴与不同血糖状态的伤口愈合和皮肤状况有关

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Type 2 diabetes affects over 340 million people worldwide. This condition can go unnoticed and undiagnosed for years, leading to a late stage where high glycaemia produces complications such as delayed wound healing. Studies have shown that 12-HHT through BLT2, accelerates keratinocyte migration and wound healing. Additionally, evidence has shown the role of nitric oxide as a pro-regenerative mediator, which is decreased in diabetes. Our main goal was to study the association between the 12-HHT/BLT2 axis and the nitric oxide production in wound healing under different glycaemia conditions. For that purpose, we used in vivo and in vitro models. Our results show that the skin from diabetic mice showed reduced BLT2 and iNOS mRNA, TEER, 12-HHT, nitrites, and tight junction levels, accompanied by higher MMP9 mRNA levels. Furthermore, a positive correlation between BLT2 mRNA and nitrites was observed. In vitro, HaCaT-BLT2 cells showed higher nitric oxide and tight junction levels, and reduced MMP9 mRNA levels, compared to mock-keratinocytes under low and high glucose condition. The wound healing capacity was associated with higher nitric oxide production and was affected by the NOS inhibition. We suggest that the BLT2 expression improves the keratinocyte response to hyperglycaemia, associated with the production of nitric oxide.
机译：2型糖尿病影响全球超过3.4亿人。这种状况可能多年未引起注意和诊断，导致血糖过高导致并发症（例如伤口愈合延迟）的晚期。研究表明，通过BLT2进行12-HHT可以加速角质形成细胞迁移和伤口愈合。另外，有证据表明一氧化氮作为促再生介质的作用，在糖尿病中减少。我们的主要目标是研究在不同血糖条件下伤口愈合中12-HHT / BLT2轴与一氧化氮生成之间的关系。为此，我们使用了体内和体外模型。我们的结果表明，糖尿病小鼠的皮肤显示出BLT2和iNOS mRNA，TEER，12-HHT，亚硝酸盐和紧密连接水平降低，并伴有更高的MMP9 mRNA水平。此外，观察到BLT2 mRNA与亚硝酸盐之间呈正相关。在体外，与低和高葡萄糖条件下的模拟角质形成细胞相比，HaCaT-BLT2细胞显示出较高的一氧化氮和紧密连接水平，并降低了MMP9 mRNA水平。伤口愈合能力与更高的一氧化氮产生有关，并受NOS抑制的影响。我们建议，BLT2的表达可改善角化细胞对高血糖的反应，并产生一氧化氮。

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