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首页> 外文期刊>Fresenius environmental bulletin >PHYLOGENETIC AND MORPHOLOGICAL CHARACTERIZATION OF FUSARIUM OXYSPORUM F. SP. CEPAE THE CAUSAL AGENT OF BASAL ROT ON ONION ISOLATED FROM TURKEY
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PHYLOGENETIC AND MORPHOLOGICAL CHARACTERIZATION OF FUSARIUM OXYSPORUM F. SP. CEPAE THE CAUSAL AGENT OF BASAL ROT ON ONION ISOLATED FROM TURKEY

机译：镰孢镰刀菌（F. SP。）的系统发育和形态特征。从土耳其分离的洋葱上剥去基腐根的诱因

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Fusarium oxysporum f. sp. cepae (FOC) is a serious pathogen, which causes basal rot on onion. In the present study, a total number of 152 Fusarium strains were isolated from infected onions picked from middle black sea region of Turkey. Based on the mean disease severity (DS %), the virulence of each isolate was recorded as highly virulent (70-100% DS), moderately virulent (2069% DS), low virulent (10-19% DS). At the morphological characterization all of high virulence Fusarium strains was identified as Fusarium ox- ysporum which based on structures of microconidia (2.5-15μmх2-3 μm and 0-septate), macroconidia (15-20 μm x 2.5-3μm and 3-septate), chlamydo- spores and other morphological characters. Phylo- genetically characterization of strains used primers ITS1 and ITS4. These primers have been successfully amplified a specific 330-bp PCR product the internal transcribed spacer (ITS1-5.8S-ITS2) region of FOC strains. At the phylogenetic classification of high virulence FOC strains Neighbor-joining method was used. There was very little genetic differences (99% similarity) between strains and reference FOC (HQ658965) NCBI Gen Bank due to environmental conditions. This isolates probably from the same clonal lineage. On the other hand, at the phylogenetic characterization, FOC strains formed major genetic differences with other pathogens on onion. Thus, the results indicate that molecular profiling using ITS is a valid method for phylogenetic characterization of FOC.
机译：尖孢镰刀菌f。 sp。 cepae（FOC）是一种严重的病原体，会导致洋葱基腐。在本研究中，从土耳其中部黑海地区采摘的受感染洋葱中共分离出152个镰刀菌菌株。根据平均疾病严重程度（DS％），每个分离株的毒力记录为高毒力（70-100％DS），中毒（2069％DS），低毒力（10-19％DS）。在形态学特征上，所有高毒镰刀菌菌株均被鉴定为镰孢镰刀菌，其基于微分生孢子（2.5-15μmх2-3μm和0隔室），大分生孢子（15-20μmx2.5-3μm和3隔室）的结构），衣原体孢子和其他形态特征。使用引物ITS1和ITS4对菌株进行植物遗传学表征。这些引物已成功扩增了FOC菌株内部转录间隔区（ITS1-5.8S-ITS2）区域的特定330 bp PCR产物。在高毒力FOC菌株的系统发育分类中，使用了Neighbor-joining方法。由于环境条件的影响，菌株与参考FOC（HQ658965）NCBI Gen Bank之间的遗传差异很小（99％相似性）。这可能与同一克隆谱系分离。另一方面，在系统发育特征上，FOC菌株与洋葱上的其他病原体形成了主要的遗传差异。因此，结果表明，使用ITS进行分子谱分析是FOC系统发育表征的有效方法。

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