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Leptin increases mitotic index and regeneration ratio in hepatectomized rats

机译：瘦素提高肝切除大鼠的有丝分裂指数和再生率

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Background The aim of this study was to evaluate the potential effect of intraperitoneal administration of leptin on the hepatic regeneration and the mitotic index. Material and Methods 56 Sprague-Dawley rats were divided into 7 groups each containing 8 rats. Group 1 was evaluated as the sham group and no surgical procedure was performed on animals. The rats in groups 2, 3, and 4 (named C24, C48, C72, respectively) were given intraperitoneal injection of 2 ml/kg normal saline 60 minutes before the surgical procedure consisting of laparotomy and 70% hepatectomy. These groups were used as controls at 24, 48, and 72 hours. The rats in groups 5, 6, and 7 (named L24, L48, and L72, respectively) were given intraperitoneal injection of 20 μg/kg doses of recombinant mouse leptin 60 minutes before the same surgical procedure. These groups were evaluated as the experiment groups at 24, 48, and 72 hours. Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI). The weights of the remaining livers were also noted. Results The values of AST and ALT were higher in the groups that were administered leptin and they had significantly higher mitotic index than the other groups. Leptin also significantly increased the regeneration ratio as compared to the control group. The weights of the remaining livers were also higher in the leptin groups. Conclusions Intraperitoneal administration of leptin was observed to increase liver regeneration and mitotic rate in 70% hepatectomized rats.
机译：背景技术这项研究的目的是评估腹膜内施用瘦素对肝再生和有丝分裂指数的潜在影响。材料与方法将56只Sprague-Dawley大鼠分为7组，每组8只。将第1组评估为假手术组，并且不对动物进行外科手术。第2、3和4组的大鼠（分别命名为C24，C48，C72）在开腹手术和70％肝切除术手术前60分钟腹膜内注射2 ml / kg生理盐水。这些组在24、48和72小时用作对照。在第5、6和7组中的大鼠（分别命名为L24，L48和L72）在相同的手术步骤之前60分钟进行腹膜内注射20μg/ kg剂量的重组小鼠瘦蛋白。将这些组分别在24、48和72小时作为实验组进行评估。收集血样中的天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT），并获取其余组织样品的肝组织病理学，再生率和有丝分裂指数（MI）。还记录了其余肝脏的重量。结果瘦素组的AST和ALT值较高，有丝分裂指数明显高于其他组。与对照组相比，瘦素还显着提高了再生率。瘦素组中其余肝脏的重量也较高。结论腹膜内施用瘦素可提高70％肝切除大鼠的肝再生和有丝分裂率。

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