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首页> 外文期刊>Medical Science Monitor Basic Research >Plumbagin induces the apoptosis of human tongue carcinoma cells through the mitochondria-mediated pathway
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Plumbagin induces the apoptosis of human tongue carcinoma cells through the mitochondria-mediated pathway

机译：Plumbagin通过线粒体介导的途径诱导人舌癌细胞的凋亡

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Background Plumbagin, a quinonoid constituent isolated from the root of Plumbago zeylanica L., has been proven to possess anti-tumor activity both in vitro and in vivo. However, its anti-tumor properties for human tongue carcinoma have not been reported. This study aimed to investigate the inhibitory effect and the underlying mechanism of plumbagin on the growth of human tongue carcinoma cells. Material and Methods Cell proliferation ability was detected by EdU incorporation assay and colony formation assay. Cell-cycle distribution was determined by flow cytometric analysis using propidium iodide (PI) staining. Cellular apoptosis was then evaluated by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Western blotting was applied to assay the expression of Bax and Bcl-2. Results Plumbagin inhibited the growth and proliferation of Tca8113 cells in vitro in a concentration- and time-dependent manner. The cell cycles of plumbagin-treated Tca8113 cells were arrested at the G2/M phase. Cells treated with plumbagin presented the characteristic morphological changes of apoptosis. The ratio of Bax/Bcl-2 was raised by plumbagin in a concentration-dependent manner. Conclusions These results indicate that plumbagin induces the apoptosis of Tca8113 cells through mitochondria-mediated pathway.
机译：背景技术已经证实，从玉米花的根中分离出的醌类成分Plumbagin在体外和体内均具有抗肿瘤活性。然而，尚未报道其对人舌癌的抗肿瘤特性。本研究旨在探讨铅肽对人舌癌细胞生长的抑制作用及其潜在机制。材料与方法通过EdU掺入法和集落形成法检测细胞的增殖能力。细胞周期分布是通过使用碘化丙啶（PI）染色的流式细胞仪分析确定的。然后通过流式细胞术和末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）分析评估细胞凋亡。 Western blotting用于检测Bax和Bcl-2的表达。结果Plumbagin在体外以浓度和时间依赖性方式抑制Tca8113细胞的生长和增殖。经李子素处理的Tca8113细胞的细胞周期被阻滞在G2 / M期。李子苷处理的细胞表现出凋亡的特征性形态变化。李子苷以浓度依赖的方式提高了Bax / Bcl-2的比例。结论这些结果表明，铅蛋白通过线粒体介导的途径诱导Tca8113细胞凋亡。

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