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首页> 外文期刊>Maxillofacial Plastic and Reconstructive Surgery >Effect of bFGF and fibroblasts combined with hyaluronic acid-based hydrogels on soft tissue augmentation: an experimental study in rats
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Effect of bFGF and fibroblasts combined with hyaluronic acid-based hydrogels on soft tissue augmentation: an experimental study in rats

机译：bFGF和成纤维细胞联合透明质酸水凝胶对软组织增生的影响：在大鼠中的实验研究

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Abstract BackgroundHyaluronic acid (HA) has been applied as a primary biomaterial for temporary soft tissue augmentation and as a carrier for cells and the delivery of growth factors to promote tissue regeneration. Although HA derivatives are the most versatile soft tissue fillers on the market, they are resorbed early, within 3 to 12?months. To overcome their short duration, they can be combined with cells or growth factors. The purpose of this study was to investigate the stimulating effects of human fibroblasts and basic fibroblast growth factors (bFGF) on collagen synthesis during soft tissue augmentation by HA hydrogels and to compare these with the effects of a commercial HA derivative (Restylane?).MethodsThe hydrogel group included four conditions. The first condition consisted of hydrogel (H) alone as a negative control, and the other three conditions were bFGF-containing hydrogel (HB), human fibroblast-containing hydrogel (HF), and human fibroblast/bFGF-containing hydrogel (HBF). In the Restylane? group (HGF), the hydrogel was replaced with Restylane? (R, RB, RF, RBF). The gels were implanted subdermally into the back of each nude mouse at four separate sites. Twelve nude mice were used for the hydrogel ( n =?6) and Restylane? groups ( n =?6). The specimens were harvested 8?weeks after implantation and assessed histomorphometrically, and collagen synthesis was evaluated by RT-PCR.ResultsThe hydrogel group showed good biocompatibility with the surrounding tissues and stimulated the formation of a fibrous matrix. HBF and HF showed significantly higher soft tissue synthesis compared to H ( p ?0.05), and human collagen type I was well expressed in HB, HF, and HBF; HBF showed the strongest expression. The Restylane? filler was surrounded by a fibrous capsule without any soft tissue infiltration from the neighboring tissue, and collagen synthesis within the Restylane? filler could not be observed, even though no inflammatory reactions were observed.ConclusionThis study revealed that HA-based hydrogel alone or hydrogel combined with fibroblasts and/or bFGF can be effectively used for soft tissue augmentation.
机译：摘要背景透明质酸（HA）已被用作临时性软组织增强的主要生物材料，并作为细胞的载体和生长因子的输送以促进组织再生。尽管HA衍生物是市场上用途最广泛的软组织填充物，但它们会在3至12个月内被早期吸收。为了克服它们的持续时间短，它们可以与细胞或生长因子结合。这项研究的目的是研究人类成纤维细胞和碱性成纤维细胞生长因子（bFGF）对HA水凝胶增强软组织过程中胶原合成的刺激作用，并将其与市售HA衍生物（Restylane？）的作用进行比较。水凝胶组包括四个条件。第一个条件包括单独的水凝胶（H）作为阴性对照，其他三个条件是含bFGF的水凝胶（HB），含人成纤维细胞的水凝胶（HF）和含人成纤维细胞/ bFGF的水凝胶（HBF）。在Restylane中？ （HGF）组，水凝胶被Restylane代替？ （R，RB，RF，RBF）。将凝胶皮下植入四个裸露部位的每只裸鼠的背部。将十二只裸鼠用于水凝胶（n =？6）和Restylane?。组（n =？6）。植入后8周，收集标本并进行组织形态学评估，并通过RT-PCR评估胶原蛋白的合成。结果水凝胶组与周围组织具有良好的生物相容性，并刺激了纤维基质的形成。与H相比，HBF和HF表现出明显更高的软组织合成（p <？0.05），并且在HB，HF和HBF中人I型胶原表达良好。 HBF显示最强的表达。 Restylane？填充物被纤维囊包裹，周围组织没有任何软组织浸润，并且Restylane®中的胶原蛋白合成即使未观察到炎症反应，也无法观察到填充物。结论：这项研究表明，单独使用HA基水凝胶或将水凝胶与成纤维细胞和/或bFGF结合可以有效地用于软组织增强。

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