• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Medical Gas Research >Oxybiotest project: microorganisms under pressure. Hyperbaric oxygen (HBO) and simple pressure interaction on selected bacteria
【24h】

Oxybiotest project: microorganisms under pressure. Hyperbaric oxygen (HBO) and simple pressure interaction on selected bacteria

机译：Oxybiotest项目：压力下的微生物。高压氧（HBO）和对选定细菌的简单压力相互作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background HyperBaric Oxygen (HBO) therapy involves exposure to pure oxygen in a pressurized room, and it is an already well-established treatment for various conditions, including those originated by serious infections. Starting from the observation of an increased number of patients who were accessing our HBO units for diseases supported from concomitant multidrug-resistant microorganisms, as well as considering the evident clinical benefit and laboratory final outcome of those patients at the end of the treatment, aim of our study was to measure, or better define at least, if there was any interaction between a hyperbaric environment and some selected microorganisms and if those positive results were due to the increased oxygen partial pressure (pO2) value or just to the increased pressure, regardless of the fraction of inspired oxygen (FiO2) applied (21÷100%). Design and methods We applied various increased pO2 values in a hyperbaric environment. Our study design was tailored in four steps to answer four specific questions, ordered in a progressive process: OxyBioTest (OBT)-1,2,3, and 4. Specifically, we chose to investigate possible changes in the Minimum Inhibitory Concentration (MIC) and in the Minimum Bactericidal Concentration (MBC) of multi-resistant microorganisms after a single session of hyperbaric therapy. Results OBT-1 and OBT-2 provide a semi-quantitative confirmation of the bacterio-cidal and cytostatic effects of HBO. HBO is cidal only if the total exposure pressure is elevated, and cidal or cytostatic effect are not always dependent on the pO2 applied. OBT-4 has shown the adjuvant effect of HBO and antimicrobial drug against some selected bacteria. Discussion We seem allowed to hypothesize that only in case of a good approach to a lesion, permitting smaller bacterial loads thanks to surgical debridement and/or eventual antibiotic therapy for example, You can observe the clear effectiveness of the HyperBaric Oxygen (HBO) exposure as a valid adjuvant therapy, even when that lesion is substained from multidrug-resistant micro-organisms. On the contrary when the bacterial load is very high we observe an unchanged situation or a just a slightly diminishing in the number of cfu/ml. Conclusions Even if confined in this ‘in vitro’ environment and in a single treatment, just knowing the microorganism strain responsible of the lesion we seem allowed to both weight the possible related effectiveness using HBO Therapy (HBOT) and derive the best pO2 to treat the case. A further possible development of the study highlights a comparison between Acinetobacter baumannii (ACBA) and Pseudomonas aeruginosa (PSAE), and Escherichia coli (ESCO) and Klebsiella pneumoniae (KLPN).
机译：背景技术高压氧（HBO）治疗涉及在加压室内暴露于纯氧，并且对于各种疾病，包括由严重感染引起的疾病，这已经是公认的治疗方法。从观察到越来越多的患者进入我们的HBO单元以治疗伴随有多种耐药性微生物的疾病开始，并考虑到这些患者在治疗结束时明显的临床获益和实验室最终结果，旨在我们的研究是测量或至少确定高压环境与某些选定的微生物之间是否存在相互作用，以及这些积极结果是否是由于氧分压（pO 2 ）值升高所致。或只是增加压力，而不考虑施加的吸入氧气（FiO 2 ）的比例（21÷100％）。设计和方法我们在高压环境中应用了各种增加的pO 2 值。我们的研究设计按四个步骤定制，以回答四个特定问题，并逐步进行排序：OxyBioTest（OBT）-1,2,3和4。特别是，我们选择研究最小抑菌浓度（MIC）的可能变化一次高压治疗后，多重耐药微生物的最低杀菌浓度（MBC）。结果OBT-1和OBT-2提供了HBO杀菌和抑制细胞作用的半定量证实。仅当总暴露压力升高时，HBO才具有杀灭作用，并且杀灭作用或抑制细胞生长的作用并不总是取决于所施加的pO 2 。 OBT-4已显示出HBO和抗菌药物对某些选定细菌的佐剂作用。讨论我们似乎可以假设，只有在对病变良好的处理方法的情况下，才能通过外科手术清创术和/或最终的抗生素治疗来实现较小的细菌负荷，例如，您可以观察到高压氧（HBO）暴露具有明显的有效性一种有效的辅助疗法，即使该病灶由耐多药微生物掩盖也是如此。相反，当细菌负荷很高时，我们观察到情况不变或cfu / ml的数量略有减少。结论即使限制在这种“体外”环境中，并且仅采用一次治疗，只要知道了造成病变的微生物菌株，我们似乎就可以通过HBO治疗（HBOT）权衡可能的相关功效，并得出最佳的pO 2 处理此案。这项研究的进一步发展突出了鲍曼不动杆菌（ACBA）和铜绿假单胞菌（PSAE）以及大肠杆菌（ESCO）和肺炎克雷伯菌（KLPN）之间的比较。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号