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Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome

机译：室温下长时间的血液培养和延长的血清存储量对人血清代谢组的影响

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Metabolomics is a powerful technology with broad applications in life science that, like other -omics approaches, requires high-quality samples to achieve reliable results and ensure reproducibility. Therefore, along with quality assurance, methods to assess sample quality regarding pre-analytical confounders are urgently needed. In this study, we analyzed the response of the human serum metabolome to pre-analytical variations comprising prolonged blood incubation and extended serum storage at room temperature by using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) -based metabolomics. We found that the prolonged incubation of blood results in a statistically significant 20% increase and 4% decrease of 225 tested serum metabolites. Extended serum storage affected 21% of the analyzed metabolites (14% increased, 7% decreased). Amino acids and nucleobases showed the highest percentage of changed metabolites in both confounding conditions, whereas lipids were remarkably stable. Interestingly, the amounts of taurine and O -phosphoethanolamine, which have both been discussed as biomarkers for various diseases, were 1.8- and 2.9-fold increased after 6 h of blood incubation. Since we found that both are more stable in ethylenediaminetetraacetic acid (EDTA) blood, EDTA plasma should be the preferred metabolomics matrix.
机译：代谢组学是一项强大的技术，在生命科学中具有广泛的应用，与其他组学方法一样，它需要高质量的样品才能获得可靠的结果并确保可重复性。因此，除了质量保证之外，迫切需要用于评估有关分析前混杂因素的样品质量的方法。在这项研究中，我们通过使用气相色谱-质谱（GC-MS）和液相色谱-串联质谱（LC）分析了人类血清代谢组对分析前变异的响应，包括长时间的血液培养和室温下延长的血清存储-MS / MS）的代谢组学。我们发现，长时间的血液孵育会导致225种经测试的血清代谢物具有统计学意义的20％的增加和4％的减少。延长的血清存储影响了21％的分析代谢产物（增加了14％，减少了7％）。在两种混杂条件下，氨基酸和核碱基均显示出最高的代谢物变化百分比，而脂质则非常稳定。有趣的是，牛磺酸和O-磷酸乙醇胺的量（已被讨论为各种疾病的生物标记物）在血液孵育6小时后分别增加了1.8倍和2.9倍。由于我们发现两者在乙二胺四乙酸（EDTA）血液中都更稳定，因此EDTA血浆应成为首选的代谢组学基质。

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