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A Comprehensive Plasma Metabolomics Dataset for a Cohort of Mouse Knockouts within the International Mouse Phenotyping Consortium

机译：国际小鼠表型研究联盟内的一组小鼠基因敲除的综合血浆代谢组学数据集。

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Mouse knockouts facilitate the study ofgene functions. Often, multiple abnormal phenotypes are induced when a gene is inactivated. The International Mouse Phenotyping Consortium (IMPC) has generated thousands of mouse knockouts and catalogued their phenotype data. We have acquired metabolomics data from 220 plasma samples from 30 unique mouse gene knockouts and corresponding wildtype mice from the IMPC. To acquire comprehensive metabolomics data, we have used liquid chromatography (LC) combined with mass spectrometry (MS) for detecting polar and lipophilic compounds in an untargeted approach. We have also used targeted methods to measure bile acids, steroids and oxylipins. In addition, we have used gas chromatography GC-TOFMS for measuring primary metabolites. The metabolomics dataset reports 832 unique structurally identified metabolites from 124 chemical classes as determined by ChemRICH software. The GCMS and LCMS raw data files, intermediate and finalized data matrices, R-Scripts, annotation databases, and extracted ion chromatograms are provided in this data descriptor. The dataset can be used for subsequent studies to link genetic variants with molecular mechanisms and phenotypes.
机译：敲除小鼠有助于基因功能的研究。当基因失活时，通常会诱发多种异常表型。国际小鼠表型研究协会（IMPC）已产生了数千个小鼠基因敲除并对其表型数据进行了分类。我们已经从30个独特的小鼠基因敲除物中的220个血浆样品以及来自IMPC的相应野生型小鼠中获取了代谢组学数据。为了获得全面的代谢组学数据，我们已使用液相色谱（LC）结合质谱（MS）来以非目标方法检测极性和亲脂性化合物。我们还使用了针对性的方法来测量胆汁酸，类固醇和脂蛋白。此外，我们已使用气相色谱GC-TOFMS测量主要代谢产物。代谢组学数据集报告了由ChemRICH软件确定的来自124个化学类别的832种独特的结构鉴定代谢物。该数据描述符中提供了GCMS和LCMS原始数据文件，中间和最终数据矩阵，R脚本，注释数据库以及提取的离子色谱图。该数据集可用于后续研究，以将遗传变异与分子机制和表型联系起来。

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