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Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia-Romagna Region (Italy) by qPCR

机译:鸟分枝杆菌亚种的估计。通过qPCR检测艾米利亚-罗马涅地区(意大利)的散装罐装牛奶中的副结核病负荷

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Abstract Consumption of milk and dairy products is considered one of the main routes of human exposure to Mycobacterium avium subsp. paratuberculosis (MAP). Quantitative data on MAP load in raw cows?¢???? milk are essential starting point for exposure assessment. Our study provides this information on a regional scale, estimating the load of MAP in bulk tank milk (BTM) produced in Emilia-Romagna region (Italy). The survey was carried out on 2934 BTM samples (88.6% of the farms herein present) using two different target sequences for qPCR (f57 and IS900). Data about the performances of both qPCRs are also reported, highlighting the superior sensitivity of IS900-qPCR. Seven hundred and eighty-nine samples tested MAP-positive (apparent prevalence 26.9%) by IS900 qPCR. However, only 90 of these samples were quantifiable by qPCR. The quantifiable samples contained a median load of 32.4 MAP cells mL ?¢????1 (and maximum load of 1424 MAP cells mL ?¢????1 ). This study has shown that a small proportion (3.1%) of BTM samples from Emilia-Romagna region contained MAP in excess of the limit of detection (1.5 ???? 10 1 MAP cells mL ?¢????1 ), indicating low potential exposure for consumers if the milk subsequently undergoes pasteurization or if it is destined to typical hard cheese production.
机译:摘要牛奶和乳制品的消费被认为是人类接触鸟分枝杆菌亚种的主要途径之一。副结核病(MAP)。生奶牛的MAP负荷定量数据?牛奶是接触评估的重要起点。我们的研究在区域范围内提供了此信息,以估算在意大利艾米利亚-罗马涅地区生产的散装罐装牛奶(BTM)中MAP的负荷。使用两个不同的qPCR靶序列(f57和IS900)对2934个BTM样品(本文中有88.6%的农场)进行了调查。还报道了有关两种qPCR性能的数据,突出了IS900-qPCR的优越灵敏度。 IS900 qPCR检测了798例MAP阳性(表观患病率26.9%)。但是,这些样品中只有90个可通过qPCR定量。可定量样品的平均负荷为32.4个MAP细胞mL -1(和最大负荷为1424个MAP细胞mL -1)。这项研究表明,来自艾米利亚—罗马涅地区的一小部分BTM样品(3.1%)含有的MAP超过了检测限(1.5 ???? 10 1 MAP细胞mL ??????? 1),表明如果牛奶随后进行了巴氏消毒,或者注定要用于典型的硬奶酪生产,则对消费者的潜在暴露量较低。

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