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A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant ?

机译:使用市售消毒剂在本科实验室进行噬菌体概念教学的简单练习?

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Virus and bacteriophage are key components in any microbiology teaching. We have used a commercially available disinfectant (TRIGENE) to demonstrate plaques formation from bacteriophages on confluent bacterial lawns. We have designed a laboratory exercise on phage typing using these plaques in a single practical class to teach the concepts of bacteria-virus interactions, specificity, and diversity in a second year biomedical science course. The artificial bacteriophage-plaques are economical and capable of reliably mimicking bacteriophage effects. Use of the disinfectant to mimick bacteriophage effects eliminated the need to source and reproduce real bacteriophages, enabling fast preparation and demonstration of plaque assay in an undergraduate laboratory. Our phage typing teaching exercise facilitated effective students’ learning about bacteriophages with minimal laboratory skills or instructor intervention.
机译:病毒和噬菌体是任何微生物学教学中的关键成分。我们已经使用市售的消毒剂(TRIGENE)来证明汇合细菌草坪上噬菌体形成的噬菌斑。我们已经在单个实际班级中设计了使用这些噬菌斑进行噬菌体分型的实验室练习,以在第二年的生物医学科学课程中教授细菌-病毒相互作用,特异性和多样性的概念。人工噬菌斑是经济的并且能够可靠地模仿噬菌体作用。使用消毒剂来模仿噬菌体的效果消除了对真正噬菌体的来源和繁殖的需要,从而可以在大学实验室中快速制备和演示噬菌斑测定法。我们的噬菌体分型教学练习以最少的实验室技能或讲师干预,帮助有效的学生学习噬菌体。

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