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首页> 外文期刊>MicrobiologyOpen >Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing
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Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing

机译：通过16S rRNA测序确定五个地区藏鸡盲肠菌群。

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Abstract Tibetan Chickens should have unique gastrointestinal microbiota because of their particular habitats. Thus, the aim of this study was to investigate the cecal microbiota of Tibetan Chickens from five typical high-altitude regions of China. Lohmann egg-laying hens (LMs) and Daheng broiler chickens (DHs) were chosen as controls. The cecal bacterial populations of Tibetan Chickens were surveyed by high-throughput sequencing (HTS) of the bacterial 16S rRNA hypervariable region V3-V4 (16S rRNAV3-V4) combined with community-fingerprinting analysis of the 16S rRNA gene based on polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results revealed that the majority of cecal microbiota differed between the Tibetan Chicken and LM/DH. The microbial communities in the cecum were composed of 16 phyla, 28 classes, 36 orders, 57 families, 101 genera, and 189 species. Represented phyla were Bacteroidetes (>47%), Firmicutes (>18.8%), Spirochaetae (>0.3%), and Proteobacteria (>0.4%). Bacteroides and the RC9 gut group were the two most abundant genera. There were relatively more Christensenellaceae , Subdoligranulum , Spirochaeta , and Treponema in Tibetan Chickens, whereas there were more Phascolarctobacterium , Faecalibacterium , Megamonas , and Desulfovibrio in LMs and DHs. The cecal microbiota of Tibetan Chicken have slightly diverged due to exposure to different geographic environments. Differences in the intestinal bacterial communities of Tibetan Chicken and LM/DH were noted.
机译：摘要藏鸡由于其特殊的生境而应具有独特的胃肠道菌群。因此，本研究的目的是调查来自中国五个典型高海拔地区的藏鸡的盲肠菌群。选择Lohmann产蛋母鸡（LMs）和大亨肉鸡（DHs）作为对照。通过对细菌16S rRNA高变区V3-V4（16S rRNAV3-V4）进行高通量测序（HTS），并基于聚合酶链反应对16S rRNA基因进行社区指纹分析，对藏鸡的盲肠细菌种群进行了调查。变性梯度凝胶电泳（PCR-DGGE）。结果表明，大多数盲肠菌群在藏鸡和LM / DH之间存在差异。盲肠中的微生物群落由16个门，28个类别，36个科，57个科，101属和189种组成。代表菌群是拟杆菌（> 47％），硬毛菌（> 18.8％），螺旋藻（> 0.3％）和变形杆菌（> 0.4％）。拟杆菌属和RC9肠道组是两个最丰富的属。藏族鸡中的克里斯滕氏菌，肉芽孢杆菌，螺旋藻和螺旋体相对较多，而LMs和DHs中的杆菌杆菌，费卡杆菌，巨型杆菌和脱硫弧菌较多。由于暴露于不同的地理环境，藏鸡的盲肠菌群略有不同。注意到藏鸡和LM / DH肠道细菌群落的差异。

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