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首页> 外文期刊>MicrobiologyOpen >Characterization of the interactions between Escherichia coli receptors, LPS and OmpC, and bacteriophage T4 long tail fibers
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Characterization of the interactions between Escherichia coli receptors, LPS and OmpC, and bacteriophage T4 long tail fibers

机译：表征大肠杆菌受体，LPS和OmpC与噬菌体T4长尾纤维之间的相互作用

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Abstract Bacteriophages have strict host specificity and the step of adsorption is one of key factors for determining host specificity. Here, we systematically examined the interaction between the Escherichia coli receptors lipopolysaccharide (LPS) and outer membrane protein C (OmpC), and the long tail fibers of bacteriophage T4. Using a variety of LPS mutants, we demonstrated that T4 has no specificity for the sugar sequence of the outer core (one of three LPS regions) in the presence of OmpC but, in the absence of OmpC, can adsorb to a specific LPS which has only one or two glucose residues without a branch. These results strengthen the idea that T4 adsorbs to E. coli via two distinct modes, OmpC-dependent and OmpC-independent, suggested by previous reports (Prehm et al. 1976; Yu and Mizushima 1982). Isolation and characterization of the T4 mutants Nik ( N o i nfection to K -12 strain), Nib ( N o i nfection to B strain), and Arl ( a ltered r ecognition of L PS) identified amino acids of the long tail fiber that play important roles in the interaction with OmpC or LPS, suggesting that the top surface of the distal tip head domain of T4 long tail fibers interacts with LPS and its lateral surface interacts with OmpC.
机译：摘要噬菌体具有严格的宿主特异性，吸附步骤是决定宿主特异性的关键因素之一。在这里，我们系统地检查了大肠杆菌受体脂多糖（LPS）和外膜蛋白C（OmpC）与噬菌体T4的长尾纤维之间的相互作用。使用多种LPS突变体，我们证明了在存在OmpC的情况下T4对外部核心（三个LPS区域之一）的糖序列没有特异性，但是在没有OmpC的情况下，它可以吸附到具有只有一个或两个葡萄糖残基没有分支。这些结果加强了T4通过两种不同的模式吸附于大肠杆菌的想法，即依赖于OmpC和不依赖于OmpC的模式，这是以前的报道所建议的（Prehm等人，1976; Yu和Mizushima，1982）。 T4突变体Nik（对K -12株的新感染），Nib（对B株的新感染）和Arl（L PS的识别）的分离和鉴定，鉴定了长尾纤维的氨基酸在与OmpC或LPS相互作用中起着重要的作用，这表明T4长尾纤维的远端头域的顶表面与LPS相互作用，而其侧面与OmpC相互作用。

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