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首页> 外文期刊>Journal of bacteriology >A component of the side tail fiber of Escherichia coli bacteriophage lambda can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage T4.
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A component of the side tail fiber of Escherichia coli bacteriophage lambda can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage T4.

机译：大肠杆菌噬菌体λ的侧尾纤维的成分可以在功能上代替无关的噬菌体T4的长尾纤维蛋白的受体识别部分。

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The distal part of the long tail fiber of Escherichia coli bacteriophage T4 consists of a dimer of protein 37. Dimerization requires the catalytic action of protein 38, which is encoded by T4 and is not present in the virion. It had previously been shown that gene tfa of the otherwise entirely unrelated phage lambda can functionally replace gene 38. Open reading frame (ORF) 314, which encodes a protein that exhibits homology to a COOH-terminal area of protein 37, is located immediately upstream of tfa. The gene was cloned and expressed in E. coli. An antiserum against the corresponding polypeptide showed that it was present in phage lambda. The serum also reacted with the long tail fibers of phage T4 near their free ends. An area of the gene encoding a COOH-terminal region of ORF 314 was recombined, together with tfa, into the genome of T4, thus replacing gene 38 and a part of gene 37 that codes for a COOH-terminal part of protein 37. Such T4-lambda hybrids, unlike T4, required the presence of outer membrane protein OmpC for infection of E. coli B. An ompC missense mutant of E. coli K-12, which was still sensitive to T4, was resistant to these hybrids. We conclude that the ORF 314 protein represents a subunit of the side tail fibers of phage lambda which probably recognize the OmpC protein. ORF 314 was designated stf (side tail fiber). The results also offer an explanation for the very unusual fact that, despite identical genomic organizations, T4 and T2 produce totally different proteins 38. An ancestor of T4 from the T2 lineage may have picked up tfa and stf from a lambdoid phase, thus possibly demonstrating horizontal gene transfer between unrelated phage species.
机译：大肠杆菌噬菌体T4的长尾纤维的远端部分由蛋白质37的二聚体组成。二聚化需要蛋白质38的催化作用，该蛋白质由T4编码且不存在于病毒体中。以前已经证明，原本完全不相关的噬菌体λ的基因tfa可以在功能上替代基因38。开放阅读框（ORF）314位于编码上游，与蛋白质37的COOH末端区域具有同源性tfa。该基因被克隆并在大肠杆菌中表达。针对相应多肽的抗血清显示它存在于噬菌体λ中。血清还在其自由端附近与噬菌体T4的长尾纤维反应。将编码ORF 314的COOH末端区域的基因区域与tfa一起重组到T4的基因组中，从而取代了基因38和基因37的一部分，该部分编码蛋白质37的COOH末端部分。与T4不同，T4-λ杂种需要存在外膜蛋白OmpC才能感染大肠杆菌B。大肠杆菌K-12的ompC错义突变体仍然对T4敏感，因此对这些杂种具有抗性。我们得出结论，ORF 314蛋白代表噬菌体λ的侧尾纤维的一个亚基，它可能识别OmpC蛋白。 ORF 314被命名为stf（侧尾纤维）。这些结果也解释了一个非常不寻常的事实，即尽管基因组结构相同，T4和T2仍会产生完全不同的蛋白质38。T4谱系的T4祖先可能已经从lambdoid相中吸收了tfa和stf，因此可能表明无关噬菌体之间的水平基因转移。

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