M-cadherin is a reliable marker of quiescent satellite cells in mouse skeletal muscle

A Wernig; M Bone; A Irintchev; R Sch?fer; M Cullen

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M-cadherin is a reliable marker of quiescent satellite cells in mouse skeletal muscle

机译：M-钙粘着蛋白是小鼠骨骼肌中静止卫星细胞的可靠标记

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Satellite cells are responsible for growth, maintenance and repair of postnatal skeletal muscle. On the search for a specific marker molecule for the identification of satellite cells by light-microscopy, we examined M-cadherin, a Ca2+-dependent cell adhesion molecule which is present in quiescent as well as activated satellite cells but not in other cell types in skeletal muscle. To determine the frequency of M-cadherin positive satellite cells in relation to total satellite cells in intact adult mouse muscle, we evaluated every satellite cell found in spaced serial sections of single muscle fibre preparations immunostained for Mcadherin by light and electron microscopy. We could identify 50 quiescent satellite cells this way and found M-cadherin antibody label in all of them. Statistically, this indicates that 94-100% of all satellite cells express Mcadherin, thus rendering M-cadherin as a reliable marker of quiescent satellite cells in mouse muscle.

机译：卫星细胞负责产后骨骼肌的生长，维持和修复。在寻找通过光学显微镜鉴定卫星细胞的特定标记分子时，我们检查了M-钙粘着蛋白，一种钙离子依赖性的细胞粘附分子，在静止和活化的卫星细胞中均存在，但在其他细胞类型中不存在。骨骼肌。为了确定完整成年小鼠肌肉中M-cadherin阳性卫星细胞相对于总卫星细胞的频率，我们通过光学和电子显微镜评估了免疫染色了Mcadherin的单个肌肉纤维制剂的间隔连续切片中发现的每个卫星细胞。我们可以以此方式鉴定出50个静止的卫星细胞，并在所有的细胞中找到M-钙粘蛋白抗体标记。从统计学上讲，这表明94-100％的所有卫星细胞都表达Mcadherin，因此使M-cadherin成为小鼠肌肉中静止的卫星细胞的可靠标记。

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《European Journal of Translational Myology》 |2004年第3期|共8页
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A Wernig; M Bone; A Irintchev; R Sch?fer; M Cullen;
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中图分类肌肉疾病及损伤;
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1. SINGLE-CELL ANALYSIS OF REGULATORY GENE EXPRESSION IN QUIESCENT AND ACTIVATED MOUSE SKELETAL MUSCLE SATELLITE CELLS [J] . Cornelison DDW., Wold BJ. Developmental biology . 1997,第2期

机译：在静止和活化的小鼠骨骼肌卫星细胞中调节基因表达的单细胞分析
2. Single-Cell Analysis of Regulatory Gene Expression in Quiescent and Activated Mouse Skeletal Muscle Satellite Cells☆ [J] . D.D.W. Cornelison, Barbara J. Wold Developmental biology . 1997,第2期

机译：静止和激活的小鼠骨骼肌卫星细胞中调控基因表达的单细胞分析☆
3. Alterations of M-cadherin, neural cell adhesion molecule and beta-catenin expression in satellite cells during overload-induced skeletal muscle hypertrophy. [J] . Ishido M, Uda M, Masuhara M, Acta physiologica . 2006,第3期

机译：在超负荷引起的骨骼肌肥大过程中卫星细胞中M-钙粘着蛋白，神经细胞粘附分子和β-连环蛋白表达的变化。
4. Quantitative Proteomics Of Mouse Skeletal Muscles After 30 Days Of Microgravity On The BION-M1 Biosatellite [C] . Georg Tascher, Pauline Maes, Alain van Dorsselaer, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：在Bion-M1生物卫星上30天微小的小鼠骨骼肌定量蛋白质组学
5. Skeletal Muscle Satellite Cells Have a MyoD-Positive Developmental Origin and Activated MyoD-Positive Satellite Cells Maintain Their Self-renewal Capacity during Adult Muscle Regeneration. [D] . Kanisicak, Onur. 2011

机译：骨骼肌卫星细胞具有MyoD阳性发育起源，而活化的MyoD阳性卫星细胞在成年肌肉再生过程中保持其自我更新能力。
6. Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells [O] . Jonathan R. Beauchamp, Louise Heslop, David S.W. Yu, 2000

机译：Cd34和Myf5的表达定义了大多数静止的成年骨骼肌卫星细胞
7. Single-Cell Analysis of Regulatory Gene Expression in Quiescent and Activated Mouse Skeletal Muscle Satellite Cells [O] . Cornelison D.D.W., Wold Barbara J. 1997

机译：静态和激活的小鼠骨骼肌卫星细胞中调节基因表达的单细胞分析

M-cadherin is a reliable marker of quiescent satellite cells in mouse skeletal muscle

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