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首页> 外文期刊>Molecular and Cellular Biomedical Sciences: MCBS >The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells
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The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells

机译：富含血小板的血浆和L-抗坏血酸对人脂肪干细胞的形态，增殖和软骨生成能力的影响

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Background: Microtia is a congenital malformation in the external ear due to cartilage defect. Adipose-derived stem cells (ADSC) is promising cells to develop cartilage tissue engineering for microtia. In this study, we focused on proliferation and chondrogenesis of ADSC in three different media, which consist of 10% fetal bovine serum (FBS), 10% FBS with L-ascorbic acid, and 10% human platelet rich plasma (PRP). Methods: ADSC were induced to differentiate into adipocytes, chondrocyte and osteocytes. ADSC morphology, proliferation and population doubling time was compared in three different media and analysed. Observation and alcian blue staining were done every 7 days to assess chondrogenic potency of ADSC from each treatment. Results: Isolated ADSC were able to differentiate into adipocytes, osteocytes and chondrocytes. ADSC in all group have fibroblast-like morphology, but cells in 10% FBS and 10% FBS with LAA group were flattened and larger. ADSC in 10% PRP group proliferates faster than 10% FBS with and without LAA. PDT values of ADSC were 34 hours, 44 hours and 48 hours, respectively for 10% PRP, 10% FBS with LAA and 10% FBS group. Alcian blue staining revealed that ADSC in 10% FBS with LAA and 10% PRP were able to proceed to chondrogenesis when cultured time were prolong up to 21 days, but not with ADSC in 10% FBS. Conclusion: We conclude that adding 10% FBS with LAA or 10% PRP into medium culture can support proliferation and chondrogenesis of ADSC.
机译：背景：小耳畸形是由于软骨缺损导致的外耳先天性畸形。脂肪干细胞（ADSC）是有希望的细胞，可以发展软骨组织工程以减少小眼病。在这项研究中，我们集中于ADSC在三种不同的培养基中的增殖和软骨形成，其中包括10％的胎牛血清（FBS），10％的LBS和抗坏血酸FBS，以及10％的富含人血小板的血浆（PRP）。方法：诱导ADSC分化为脂肪细胞，软骨细胞和骨细胞。在三种不同的培养基中比较了ADSC的形态，增殖和种群倍增时间并进行了分析。每7天进行一次观察和阿尔辛蓝染色，以评估每次处理后ADSC的软骨生成能力。结果：分离的ADSC能够分化为脂肪细胞，骨细胞和软骨细胞。所有组的ADSC都具有成纤维细胞样的形态，但是LAA组的10％FBS和10％FBS中的细胞扁平且变大。有和没有LAA的情况下，10％PRP组的ADSC的增殖速度快于10％FBS。对于10％PRP，10％FBS（LAA）和10％FBS组，ADSC的PDT值分别为34小时，44小时和48小时。阿尔辛蓝染色显示，当培养时间延长至21天时，含LAA和10％PRP的10％FBS中的ADSC能够进行软骨形成，而不含10％FBS的ADSC中则不能进行软骨形成。结论：我们得出的结论是，在培养基中添加10％FBS和LAA或10％PRP可以支持ADSC的增殖和软骨形成。

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