Establishment of Methylation-Specific PCR for the Mouse p53 Gene

RyujiOkazaki; AkiraOotsuyama; YasuhiroYoshida; ToshiyukiNorimura

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Establishment of Methylation-Specific PCR for the Mouse p53 Gene

机译：小鼠p53基因甲基化特异性PCR的建立

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Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with both primers. In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers. We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 gene.

机译：尚未报道小鼠p53基因的甲基化特异性PCR（MSP）。我们搜索了CpG岛，对双亚硫酸化DNA进行了测序，并设计了用于甲基化和非甲基化位点的PCR引物。来自幼鼠的DNA用未甲基化的引物产生了很强的PCR产物，而用甲基化的引物产生了较弱的条带。用两只引物从一只老老鼠的DNA产生强度相似的条带。在放射线诱发的肿瘤中，一只老老鼠的DNA用两种引物产生相似的条带。我们建议MSP是小鼠p53基因的表观遗传研究的有价值的技术。

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《Molecular biology international》 |2011年第2期|共4页
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RyujiOkazaki; AkiraOotsuyama; YasuhiroYoshida; ToshiyukiNorimura;
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中图分类分子生物学;
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6. Establishment of Methylation-Specific PCR for the Mouse p53 Gene [O] . Ryuji Okazaki, Akira Ootsuyama, Yasuhiro Yoshida, 2011

机译：小鼠p53基因甲基化特异性PCR的建立
7. Establishment of Methylation-Specific PCR for the Mouse p53 Gene [O] . Okazaki, Ryuji, Ootsuyama, Akira, Yoshida, Yasuhiro, 2011

机译：小鼠p53基因甲基化特异性PCR的建立

Establishment of Methylation-Specific PCR for the Mouse p53 Gene

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