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首页> 外文期刊>Modern Instrumentation >A Low Cost and Versatile STED Superresolution Fluorescent Microscope
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A Low Cost and Versatile STED Superresolution Fluorescent Microscope

机译：低成本，多功能的STED超分辨率荧光显微镜

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摘要

A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.
机译：描述了一种通用且便宜的超分辨率荧光显微镜，其功能与常规共聚焦显微镜一样容易。将显微镜的组件设计在平台上，该平台放置在多余的显微镜框架的顶部。给出了所有使用的光学组件和设备。激发光束和耗尽光束是从紧凑的低成本超连续谱光源中提取的。研究了耗尽光束在焦平面上的焦点，并通过一个100 nm孔径的近场光纤尖端对其成像。由0.9数值孔径显微镜物镜聚焦的共线激发和耗尽光束产生90 nm的横向超分辨率，这通过对100 nm直径的荧光珠成像来验证。

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