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首页> 外文期刊>Molecular biology of the cell >The Role of Trs65 in the Ypt/Rab Guanine Nucleotide Exchange Factor Function of the TRAPP II Complex
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The Role of Trs65 in the Ypt/Rab Guanine Nucleotide Exchange Factor Function of the TRAPP II Complex

机译：Trs65在TRAPP II复合物的Ypt / Rab鸟嘌呤核苷酸交换因子功能中的作用。

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The conserved modular complex TRAPP is a guanine nucleotide exchanger (GEF) for the yeast Golgi Ypt-GTPase gatekeepers. TRAPP I and TRAPP II share seven subunits and act as GEFs for Ypt1 and Ypt31/32, respectively, which in turn regulate transport into and out of the Golgi. Trs65/Kre11 is one of three TRAPP II-specific subunits. Unlike the other two subunits, Trs120 and Trs130, Trs65 is not essential for viability, is conserved only among some fungi, and its contribution to TRAPP II function is unclear. Here, we provide genetic, biochemical, and cellular evidence for the role of Trs65 in TRAPP II function. First, like Trs130, Trs65 localizes to the trans -Golgi. Second, TRS65 interacts genetically with TRS120 and TRS130 . Third, Trs65 interacts physically with Trs120 and Trs130. Finally, trs65 mutant cells have low levels of Trs130 protein, and they are defective in the GEF activity of TRAPP II and the intracellular distribution of Ypt1 and Ypt31/32. Together, these results show that Trs65 plays a role in the Ypt GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits. Elucidation of the role played by Trs65 in intracellular trafficking is important for understanding how this process is coordinated with two other processes in which Trs65 is implicated: cell wall biogenesis and stress response.
机译：保守的模块复合物TRAPP是用于酵母高尔基Ypt-GTPase关守的鸟嘌呤核苷酸交换剂（GEF）。 TRAPP I和TRAPP II共有七个亚基，分别充当Ypt1和Ypt31 / 32的GEF，它们依次调节进出高尔基体的运输。 Trs65 / Kre11是三个TRAPP II特异亚基之一。与其他两个亚基Trs120和Trs130不同，Trs65不是生存力所必需的，仅在某些真菌中是保守的，其对TRAPP II功能的贡献尚不清楚。在这里，我们提供Trs65在TRAPP II功能中的作用的遗传，生物化学和细胞证据。首先，与Trs130一样，Trs65定位于反式高尔基体。其次，TRS65与TRS120和TRS130遗传相互作用。第三，Trs65与Trs120和Trs130物理交互。最后，trs65突变细胞的Trs130蛋白水平较低，并且它们在TRAPP II的GEF活性以及Ypt1和Ypt31 / 32的细胞内分布方面存在缺陷。总之，这些结果表明，Trs65与其他两个TRAPP II特异性亚基协同作用，在TRAPP II的Ypt GEF活性中起作用。阐明Trs65在细胞内运输中的作用对于理解此过程与牵连Trs65的其他两个过程如何协调非常重要：细胞壁生物发生和应激反应。

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