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首页> 外文期刊>Modern Pathology >Concurrent Ki-67 and p53 Immunolabeling in Cutaneous Melanocytic Neoplasms: An Adjunct for Recognition of the Vertical Growth Phase in Malignant Melanomas?
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Concurrent Ki-67 and p53 Immunolabeling in Cutaneous Melanocytic Neoplasms: An Adjunct for Recognition of the Vertical Growth Phase in Malignant Melanomas?

机译：在皮肤黑素瘤中同时进行Ki-67和p53免疫标记：恶性黑素瘤垂直生长阶段的识别辅助吗？

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Ki-67 labeling of paraffin sections has been correlated with the number of cells in non-Go phases of the replicative cell cycle, and this immunohistochemical technique has been applied to the evaluation of a variety of human neoplasms. Similarly, immunolabeling for p53 protein has been used to detect mutations in the corresponding gene, as a reflection of possible cellular transformation in the same context. Both of these techniques were applied to 253 melanocytic tumors of the skin to assess their possible utility in the diagnosis and subcategorization of such lesions. They included 76 banal (common) nevi (CN), 39 Spitz nevi (SN), 62 superficial spreading malignant melanomas in radial growth (SSMMs), 32 nodular malignant melanomas (NMMs), 21 lentigo maligna melanomas in radial growth (LMMs), and 23 melanomas arising in association with preexisting compound nevi (MCN). One hundred cells were counted randomly in each tumor, and dark, exclusively nuclear reactivity was scored as positive labeling; results were recorded as percentages. Negligible Ki-67 and p53 labeling was seen in CN and SN, at a level that was similar to that obtained in cases of LMM and MCN. The largest proportion of Ki-67–positive and p53-positive cells was observed in NMMs, followed by SSMMs. Radial growth-phase SSMMs and LMMs demonstrated immunoprofiles that were similar to those of melanocytic nevi, and MCN did so as well. The prototypical malignant melanocytic tumor representing the vertical growth phase—nodular melanoma—demonstrated a statistically significant difference from all other lesions in this study with respect to Ki-67 index (P =.008, 2) and p53 reactivity (P 2). Subsequent concurrent use of a Ki-67 threshold index of 10% and a p53 index of 5% correctly indicated the presence of vertical growth in 75% of NMMs, whereas only 8% of radial growth phase melanomas of other types were colabeled at the same levels of reactivity for the two markers (P 2). Thus, although the distinction between benign and malignant melanocytic tumors could and should not be based on immunohistology for Ki-67 and p53, these results suggest that the latter determinants may, in fact, be used as an adjunct to morphology in the recognition of the vertical growth phase in cutaneous malignant melanomas.
机译：Ki-67标记石蜡切片已与复制性细胞周期非转染阶段的细胞数量相关联，并且这种免疫组织化学技术已应用于评估各种人类肿瘤。同样，p53蛋白的免疫标记已用于检测相应基因中的突变，以反映在相同情况下可能发生的细胞转化。将这两种技术都应用于253个皮肤黑素细胞肿瘤，以评估它们在此类病变的诊断和分类中的可能用途。其中包括76例（普通）痣（CN），39例斯皮兹痣（SN），62例放射状生长的浅表性恶性黑色素瘤（SSMM），32例恶性radial状恶性黑色素瘤（NMM），21例放射状生长的扁桃状恶性黑色素瘤（LMM），以及与先前存在的化合物痣（MCN）相关的23个黑色素瘤。在每个肿瘤中随机计数一百个细胞，并且将暗的，仅核反应性记为阳性标记。结果记录为百分比。在CN和SN中发现的Ki-67和p53标记可忽略不计，其水平与LMM和MCN的水平相似。在NMMs中观察到最大比例的Ki-67阳性和p53阳性细胞，其次是SSMMs。径向生长期SSMMs和LMMs的免疫谱与黑素细胞痣相似，而MCN也是如此。代表垂直生长期的典型恶性黑素细胞瘤（结节性黑色素瘤）与Ki-67指数（P = .008，2）和p53反应性（P 2）相比，在本研究中具有统计学上的显着差异。随后并发使用Ki-67阈值指数10 ％和p53指数5 ％正确地表明在75％的NMM中存在垂直生长，而其他类型的放射状黑色素瘤仅占8％在两种标记物的相同反应性水平上被共标记（P 2）。因此，尽管良性和恶性黑素细胞瘤的区分可以并且不应该基于Ki-67和p53的免疫组织学，但这些结果表明，后一种决定因素实际上可以用作形态学的辅助手段，以识别肿瘤细胞。皮肤恶性黑色素瘤的垂直生长期。

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