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首页> 外文期刊>Modern Pathology >Scoring of p53, VEGF, Bcl-2 and APAF-1 immunohistochemistry and interobserver reliability in colorectal cancer
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Scoring of p53, VEGF, Bcl-2 and APAF-1 immunohistochemistry and interobserver reliability in colorectal cancer

机译：大肠癌中p53，VEGF，Bcl-2和APAF-1免疫组化的评分及观察者间的信度

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Molecular tumor markers are often studied in colorectal cancer using immunohistochemistry to determine their prognostic or predictive value. Protein expression is typically assigned a 'positive' score based on a predetermined cutoff. A semiquantitative scoring method that evaluates the percentage of positive tumor cells (0–100%) may provide a better understanding of the prognostic or predictive significance of these markers. The aim of this study was to assess and compare the interobserver agreement of immunohistochemistry scores using a percentage scoring method and three categorical scoring systems. Immunohistochemistry for p53, Bcl-2, vascular endothelial growth factor (VEGF) and apoptotic protease activating factor-1 (APAF-1) was performed on 87 tumor biopsies from patients with rectal carcinoma and scored independently by four pathologists as the percentage of positive tumor cells. Interobserver agreement was assessed by the intraclass correlation coefficient. The intraclass correlation coefficients for p53 and VEGF (>0.6) indicate substantial agreement between observers. The distribution of Bcl-2 and APAF-1 scores in addition to weaker interobserver agreement by percentage scoring suggest that this approach may not be appropriate for these proteins. In conclusion, p53 and VEGF protein expression assessed by immunohistochemistry in colorectal cancer and scored as a percentage of positive tumor cells may be a viable alternative scoring method.
机译：分子肿瘤标志物经常在大肠癌中使用免疫组织化学进行研究，以确定其预后或预测价值。通常基于预定的临界值为蛋白质表达分配“阳性”评分。一种评估阳性肿瘤细胞百分比（0-100％）的半定量评分方法可以更好地了解这些标志物的预后或预测意义。这项研究的目的是评估和比较观察者间使用百分比计分方法和三个分类计分系统的免疫组化分数的一致性。对来自直肠癌患者的87例活检组织进行了p53，Bcl-2，血管内皮生长因子（VEGF）和凋亡蛋白酶激活因子1（APAF-1）的免疫组织化学分析，并由四位病理学家分别评分为阳性肿瘤百分比细胞。观察者之间的一致性通过类内相关系数进行评估。 p53和VEGF的类内相关系数（> 0.6）表明观察者之间基本一致。 Bcl-2和APAF-1分数的分布，以及较弱的观察者之间的一致性（按百分比计分）表明，这种方法可能不适用于这些蛋白质。总之，通过免疫组织化学评估结直肠癌中p53和VEGF蛋白的表达并以阳性肿瘤细胞的百分比进行评分可能是一种可行的替代评分方法。

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