...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Cancer >Correlation between centromere protein-F autoantibodies and cancer analyzed by enzyme-linked immunosorbent assay
【24h】

Correlation between centromere protein-F autoantibodies and cancer analyzed by enzyme-linked immunosorbent assay

机译：酶联免疫吸附法分析着丝粒蛋白F自身抗体与癌症的相关性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Centromere protein-F (CENP-F) is a large nuclear protein of 367?kDa, which is involved in multiple mitosis-related events such as proper assembly of the kinetochores, stabilization of heterochromatin, chromosome alignment and mitotic checkpoint signaling. Several studies have shown a correlation between CENP-F and cancer, e.g. the expression of CENP-F has been described to be upregulated in cancer cells. Furthermore, several studies have described a significant correlation between the expression of autoantibodies to CENP-F and cancer. Methods Autoantibodies to CENP-F were detected in a small number of samples during routine indirect immunofluorescence (IIF) analysis for anti-nuclear antibodies (ANA) using HEp-2 cells as substrate. Using overlapping synthetic peptides covering a predicted structural maintenance of chromosomes (SMC) domain, we developed an enzyme-linked immunosorbent assay ( ELISA ) for detection of CENP-F antibodies. Results Analyzing the reactivity of the sera positive in IIF for CENP-F antibodies to overlapping CENP-F peptides, we showed that autoantibodies to several peptides correlate with the presence of antibodies to CENP-F and a diagnosis of cancer, as increased CENP-F antibody expression specific for malignant cancer patients to five peptides was found (A9, A12, A14, A16, A27). These antibodies to CENP-F in clinical samples submitted for ANA analysis were found to have a positive predictive value for cancer of 50%. Furthermore, the expression of cancer-correlated CENP-F antibodies seemed to increase as a function of time from diagnosis. Conclusion These results conform to previous findings that approximately 50% of those patients clinically tested for ANA analyses who express CENP-F antibodies are diagnosed with cancer, confirming that these antibodies may function as circulating tumor markers. Thus, a peptide-based CENP-F ELISA focused on the SMC domain may aid in identifying individuals with a potential cancer.
机译：背景着丝粒蛋白F（CENP-F）是一种367？kDa的大核蛋白，与多种与有丝分裂有关的事件有关，例如动植物的正确装配，异染色质的稳定，染色体比对和有丝分裂检查点信号传导。几项研究表明CENP-F与癌症之间存在相关性，例如已经描述CENP-F的表达在癌细胞中被上调。此外，一些研究已经描述了针对CENP-F的自身抗体的表达与癌症之间的显着相关性。方法在以HEp-2细胞为底物的常规间接免疫荧光（IIF）分析抗核抗体（ANA）期间，在少量样品中检测到CENP-F自身抗体。我们使用覆盖了预测的染色体结构维持结构（SMC）的重叠合成肽，开发了用于检测CENP-F抗体的酶联免疫吸附测定（ELISA）。结果分析CEIF-F抗体的IIF阳性血清对重叠的CENP-F肽的反应性，我们发现针对几种肽的自身抗体与CENP-F抗体的存在和癌症诊断相关，因为CENP-F升高发现了针对恶性肿瘤患者的针对五个肽的特异性抗体表达（A9，A12，A14，A16，A27）。在提交给ANA分析的临床样本中，这些针对CENP-F的抗体被发现对癌症的阳性预测值为50％。此外，与癌症相关的CENP-F抗体的表达似乎从诊断开始就随时间增加。结论这些结果与以前的发现相符，在临床上经ANA分析临床测试的表达CENP-F抗体的患者中约有50％被诊断出患有癌症，从而证实这些抗体可以作为循环肿瘤标志物。因此，专注于SMC结构域的基于肽的CENP-F ELISA可能有助于鉴定患有潜在癌症的个体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号