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首页> 外文期刊>mSystems >Genomic Characterization and Virulence Potential of Two Fusarium oxysporum Isolates Cultured from the International Space Station
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Genomic Characterization and Virulence Potential of Two Fusarium oxysporum Isolates Cultured from the International Space Station

机译：国际空间站培养的两种尖孢镰刀菌菌株的基因组鉴定和毒力潜能

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摘要

Two isolates of Fusarium oxysporum , ISS-F3 and ISS-F4, were cultured from the dining table on the International Space Station (ISS). Genomic analyses using EF-1α sequences, presence/absence of effector proteins, k-mer comparisons, and single nucleotide polymorphisms indicate that these two strains are genomically different from 65 known sequenced strains. Functional analysis revealed that ISS-F3/F4 had higher relative abundances of polyketide synthase domains than a non-plant-pathogenic soil isolate, used for biocontrol properties (Fo47), and a clinical isolate (FOSC-3a). Putative secondary metabolite analysis indicates that ISS-F3/F4 may produce yet-unreported polyketides and nonribosomal peptides. While genomic analysis showed that these ISS strains are unlikely to be plant pathogens, a virulence assay using an immunocompromised Caenorhabditis elegans model of fusariosis revealed that they were virulent and may represent opportunistic pathogens in animals, including humans. However, its effects on the health of immunocompromised humans warrant further study. IMPORTANCE This is the first study to isolate and characterize F. oxysporum isolates from a built environment, as well as one that has been exposed to space. The characterization and analysis of these two genomes may have important implications for the medical, agricultural, and food industries as well as for the health of the crew who coinhabit the ISS with these strains.
机译：在国际空间站（ISS）的餐桌上培养了尖孢镰刀菌的两个分离株ISS-F3和ISS-F4。使用EF-1α序列的基因组分析，效应蛋白的存在与否，k-mer比较和单核苷酸多态性表明，这两个菌株在基因组上不同于65个已知测序菌株。功能分析表明，ISS-F3 / F4的聚酮化合物合酶结构域的相对丰度高于非植物致病性土壤分离物（用于生物防治特性（Fo47）和临床分离物（FOSC-3a））。推定的次级代谢产物分析表明，ISS-F3 / F4可能产生尚未报道的聚酮和非核糖体肽。虽然基因组分析表明这些ISS菌株不太可能是植物病原体，但使用免疫功能低下的镰刀菌秀丽线虫秀丽隐杆线虫模型进行的毒力测定显示，它们具有毒性，可能代表动物（包括人）的机会性病原体。但是，其对免疫受损人类健康的影响值得进一步研究。重要信息这是首次从建筑环境中以及暴露于太空的环境中分离和鉴定尖孢镰刀菌菌株的研究。这两个基因组的表征和分析可能对医学，农业和食品工业以及与这些菌株共存于国际空间站的工作人员的健康具有重要意义。

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