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首页> 外文期刊>Microbes and Environments >Population Structure of Endomicrobia in Single Host Cells of Termite Gut Flagellates ( Trichonympha spp.)
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Population Structure of Endomicrobia in Single Host Cells of Termite Gut Flagellates ( Trichonympha spp.)

机译：白蚁肠道鞭毛虫（Trichonympha spp。）单宿主细胞内微生物的种群结构。

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The gut microbiota of many phylogenetically lower termites is dominated by the cellulolytic flagellates of the genus Trichonympha , which are consistently associated with bacterial symbionts. In the case of Endomicrobia, an unusual lineage of endosymbionts of the Elusimicrobia phylum that is also present in other gut flagellates, previous studies have documented strict host specificity, leading to the cospeciation of “ Candidatus Endomicrobium trichonymphae” with their respective flagellate hosts. However, it currently remains unclear whether one Trichonympha species is capable of harboring more than one Endomicrobia phylotype. In the present study, we selected single Trichonympha cells from the guts of Zootermopsis nevadensis and Reticulitermes santonensis and characterized their Endomicrobia populations based on internal transcribed spacer (ITS) region sequences. We found that each host cell harbored a homogeneous population of symbionts that were specific to their respective host species, but phylogenetically distinct between each host lineage, corroborating cospeciation being caused by vertical inheritance. The experimental design of the present study also allowed for the identification of an unexpectedly large amount of tag-switching between samples, which indicated that any high-resolution analysis of microbial community structures using the pyrosequencing technique has to be interpreted with great caution.
机译：许多系统发育上较低的白蚁的肠道微生物区系由Trichonympha属的纤维素分解鞭毛占主导地位，其与细菌共生体一致。就内生微生物而言，其他肠道鞭毛虫中也存在非常规的内生菌壁内共生菌谱系，先前的研究已证明严格的宿主特异性，导致“三歧念珠菌内生微生物假丝酵母”与它们各自的鞭毛虫宿主共存。然而，目前尚不清楚是否有一种千金藤属物种能够携带不止一种内生菌种系统型。在本研究中，我们从新兽疫和线虫白蚁的内脏中选择了单个单毛onym属细胞，并根据内部转录间隔区（ITS）序列对它们的内微生物种群进行了表征。我们发现每个宿主细胞都拥有一个同质共生群体，这些群体特定于它们各自的宿主物种，但是每个宿主谱系在系统发育上是不同的，从而证实了由垂直遗传引起的共生。本研究的实验设计还允许鉴定样品之间出乎意料的大量标签切换，这表明使用焦磷酸测序技术对微生物群落结构进行的任何高分辨率分析都必须格外谨慎。

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