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首页> 外文期刊>Nepal Journal of Biotechnology >Detection and Quantitation of Aflatoxin for the Diagnosis of Aspergillus flavus
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Detection and Quantitation of Aflatoxin for the Diagnosis of Aspergillus flavus

机译：黄曲霉毒素的检测和定量用于黄曲霉的诊断

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Aflatoxins are the potent mycotoxins produced by Aspergillus flavus, which is hepatotoxic causing hepatocellular carcinoma. A. flavus produces sufficient amount of Aflatoxin B1 under favourable environments. Inhalation of spores and use of Aflatoxin B1, contaminated food by Aspergillus spp., could transfuse the toxins in the blood streams. The presence of these toxins in body fluid can be detected by immunological assays and which provides an effective technique for the diagnosis of the disease caused by A. flavus. Aflatoxins producing strain of A. flavus were screened in Aflatoxin Producing Medium. Production of Aflatoxin B1 by A. flavus was studied in different parameters such as incubation periods, temperatures, pH variations, sucrose concentration in Yeast Extract Sucrose medium and different natural media such as par-boiled rice, corn and groundnuts. The detection of toxins was done by TLC using silica gel (Merk) coated plates and confirmative test was done by Association of Official Analytical Chemists (AOAC) method. Presence and quantization was done by Enzyme Linked Immunosorbent Assay (ELISA) technique. Highest amount of Aflatoxin B1 was reported 68.56 ng/ml by ELISA in synthetic medium (Yeast Extract Sucrose) with 2% sucrose, pH 5.5, on 14th days of incubation, at 28±1°C (p-value 0.05). Similarly, highest amount was recorded in groundnuts (121.20ng/g) by ELISA and (500ng/kg) by TLC methods. ELISA is one of the most efficient methods used for detection and diagnosis of human diseases cause due to exposure of Aflatoxin B1 and A. flavus.Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 6-9
机译：黄曲霉毒素是黄曲霉产生的强力霉菌毒素，具有肝毒性，可引起肝细胞癌。黄曲霉在有利的环境下会产生足够量的黄曲霉毒素B1。吸入孢子和使用曲霉属菌污染的食品黄曲霉毒素B1可能会在血液中注入毒素。这些毒素在体液中的存在可以通过免疫学检测来检测，并且为诊断由黄曲霉菌引起的疾病提供了一种有效的技术。在黄曲霉产生培养基中筛选黄曲霉产生黄曲霉的菌株。在不同的参数下研究了黄曲霉产生黄曲霉毒素B1的条件，例如潜伏期，温度，pH值变化，酵母提取物蔗糖培养基中的蔗糖浓度以及不同的自然培养基，如半熟米，玉米和花生。毒素的检测是使用硅胶（Merk）包被的板通过TLC进行的，确认试验是通过官方分析化学家协会（AOAC）的方法进行的。存在和定量通过酶联免疫吸附测定（ELISA）技术完成。在ELISA的第14天，在28±1°C下，在含2％蔗糖，pH 5.5的合成培养基（酵母提取物蔗糖）中，ELISA法报告黄曲霉毒素B1的最高含量为68.56 ng / ml（p值0.05）。同样，通过ELISA在花生中的含量最高（121.20ng / g），通过薄层色谱法记录的最高含量为（500ng / kg）。 ELISA是检测和诊断因黄曲霉毒素B1和黄曲霉菌暴露而引起的人类疾病的最有效方法之一。尼泊尔生物技术杂志。 2015年12月3，编号1：6-9

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