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首页> 外文期刊>Nanomaterials >Quantum Dot Doping-Induced Photoluminescence for Facile, Label-Free, and Sensitive Pyrophosphatase Activity Assay and Inhibitor Screening
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Quantum Dot Doping-Induced Photoluminescence for Facile, Label-Free, and Sensitive Pyrophosphatase Activity Assay and Inhibitor Screening

机译：量子点掺杂诱导的光致发光，用于简便，无标记和敏感的焦磷酸酶活性测定和抑制剂筛选

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摘要

Development of simple, convenient, and sensitive assay methods for pyrophosphatase (PPase) activity is of importance, for disease diagnosis and drug discovery. Herein, a simple, rapid, label-free, and sensitive fluorescence sensor for PPase activity assay is developed, using Cu 2+ doping-induced quantum dot (QD) photoluminescence as a signal reporter. The Cu 2+ doping of ZnSe QD can induce a dopant-dependent emission response, which will be inhibited after the premixing of Cu 2+ with pyrophosphate (PPi), to form a Cu 2+ -PPi complex. Then, the hydrolysis of PPi into phosphate (Pi), specifically catalyzed by PPase, liberates the free Cu 2+ to regain the QD doping for the fluorescence response, which is highly dependent on the PPase activity. The PPase can be sensitively and selectively assayed, with a detection limit of 0.1 mU/mL. The developed sensing strategy can be also employed for the PPase inhibitor screening. Thus, the current QD doping-based sensing strategy offers an efficient and promising avenue for Cu 2+ , PPi, or PPase-related target analysis, and might hold great potential for the further applications in the clinical disease diagnosis.
机译：开发用于焦磷酸酶（PPase）活性的简单，便捷和灵敏的测定方法对于疾病诊断和药物发现至关重要。本文中，使用Cu 2+掺杂诱导的量子点（QD）光致发光作为信号报告物，开发了一种用于PPase活性测定的简单，快速，无标记且灵敏的荧光传感器。 ZnSe QD的Cu 2+掺杂可以诱导依赖掺杂剂的发射响应，在将Cu 2+与焦磷酸盐（PPi）预混合形成Cu 2+ -PPi复合物后，该响应会受到抑制。然后，PPi特异性地被PPase催化水解成磷酸盐（Pi），从而释放出游离的Cu 2+来重新获得QD掺杂的荧光响应，这在很大程度上取决于PPase的活性。可以灵敏和选择性地检测PPase，检测限为0.1 mU / mL。开发的传感策略也可用于PPase抑制剂筛选。因此，当前基于QD掺杂的传感策略为Cu 2+，PPi或PPase相关靶标分析提供了有效且有前途的途径，并可能在临床疾病诊断中具有更大的应用潜力。

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