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首页> 外文会议>Frontiers in biological detection: from nanosensors to systems VI >Label-free assay for the detection of glucose mediated by the effects of narrowband absorption on quantum dot photoluminescence
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Label-free assay for the detection of glucose mediated by the effects of narrowband absorption on quantum dot photoluminescence

机译：窄带吸收对量子点光致发光作用介导的葡萄糖无标记检测

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摘要

We present a novel strategy for label-free detection of glucose based on CdSe/ZnS core/shell quantum dots (QDs). We exploit the concentration-dependent, narrowband absorption of the hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay to selectively filter a 365-nm excitation source, leading to a proportional decrease in the photoluminescence intensity of the QDs. The visible wavelength emission of the QDs enables quantitative readout using standard visible detectors (e.g., CCD). Experimental results show highly linear QD photoluminescence over the clinically relevant glucose concentration range of 1-25mM, in excellent agreement with detection methods demonstrated by others. The method has a demonstrated limit of detection of 3.5μM, also on par with the best proposed methods. A significant advantage of our strategy is the complete elimination of QDs as a consumable. In contrast with other methods of QD-based measurement of glucose, our system does not require the glucose solution to be mixed with the QDs, thereby decreasing its overall cost and making it an ideal strategy for point-of-care detection of glucose in low-resource areas. Furthermore, readout can be accomplished with low-cost, portable detectors such as cellular phones, eliminating the need for expensive and bulky spectrophotometers to output quantitative information. The general strategy we present is useful for other biosensing applications involving chemistries with unique absorption peaks falling within the excitation band of available QDs.
机译：我们提出了一种基于CdSe / ZnS核/壳量子点（QDs）的无标签检测葡萄糖的新策略。我们利用己糖激酶-葡萄糖6-磷酸脱氢酶酶促测定的浓度依赖性，窄带吸收来选择性过滤365 nm激发源，从而导致量子点的光致发光强度成比例下降。 QD的可见光发射能够使用标准可见光检测器（例如CCD）进行定量读出。实验结果表明，在临床相关的葡萄糖浓度范围为1-25mM的情况下，高度线性的QD光致发光与其他人证明的检测方法高度吻合。该方法的检出限已证明为3.5μM，也与建议的最佳方法相当。我们策略的显着优势是完全消除了QD作为消耗品。与其他基于QD的葡萄糖测量方法相比，我们的系统不需要将葡萄糖溶液与QD混合，从而降低了总体成本，使其成为低剂量葡萄糖即时检测的理想策略资源区。此外，可以使用低成本便携式检测器（例如蜂窝电话）来完成读数，从而无需昂贵而笨重的分光光度计来输出定量信息。我们提出的一般策略对于涉及化学物质的其他生物传感应用很有用，这些化学物质的独特吸收峰落在可用量子点的激发带内。

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