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首页> 外文期刊>Nematropica >ISOLATES OF POCHONIA CHLAMYDOSPORIA VAR. CHLAMYDOSPORIA FROM MEXICO AS POTENTIAL BIOLOGICAL CONTROL AGENTS OF NACOBBUS ABERRANS
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ISOLATES OF POCHONIA CHLAMYDOSPORIA VAR. CHLAMYDOSPORIA FROM MEXICO AS POTENTIAL BIOLOGICAL CONTROL AGENTS OF NACOBBUS ABERRANS

机译：中华绒螯蟹的变种来自墨西哥的衣原体作为纳克布氏菌的潜在生物防治剂

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Perez-Rodriguez, I., A. Doroteo-Mendoza, F. Franco-Navarro, V. Santiago-Santiago and A. Montero-Pineda. 2007. Isolates of Pochonia chlamydosporia var. chlamydosporia from Mexico, as potential biological control agents of Nacobbus aberrans. Nematropica 37:127-134. Pathogenicity of five Mexican isolates of Pochonia chlamydosporia var. chlamydosporia to eggs of Nacobbus aberrans was tested. A standardized method for mass production of the fungus was established and the potential of isolates as control agents of the nematode in glasshouse production was evaluated. Three isolates (MPc1-MPc3) parasitized 77.2%-89.0% of eggs using potato agar plates as fungal inoculum, and 72.0%-87.0% using colonized rice as inoculum. In glasshouse production, tomato plants inoculated with 15,000 chlamydospores/g of soil of isolate MPc3 showed less damage and fewer nematodes in roots compared to untreated soil. Chlamydospores and colony forming units (CFU)/g of substrate colonized by MPc3 were greater after 15 days of liquid and solid fermentation in rice. Biphasic fermentation in cracked maize for 21 days allowed chlamydospore yields and numbers of CFU/g of substrate that were greater than those of the fermentation in rice, even if the percent germination of chlamydospores was less than that in rice. Chlamydospore concentration decreased in both substrates by prolonging the fermentation period to 28 days. In Mexico, cracked maize is a cheaper substrate than rice and potentially as effective as rice for mass production of the fungus. Results confirm the potential of this fungus as a biological control agent of N. aberrans.
机译：佩雷斯·罗德里格斯（Perez-Rodriguez），A。Doroteo-Mendoza，F。Franco-Navarro，V。Santiago-Santiago和A. Montero-Pineda。 2007。分离的Pochonia chlamydosporia var。来自墨西哥的衣原体，作为潜在的纳博克生物防治剂。 Nematropica 37：127-134。五种墨西哥波黑衣原体的致病性。测试了衣原体对Nacobbus aberrans卵的感染。建立了真菌大量生产的标准化方法，并评估了分离株作为温室生产中线虫防治剂的潜力。使用马铃薯琼脂平板作为真菌接种物，三种分离株（MPc1-MPc3）寄生了77.2％-89.0％的卵，使用定植的水稻作为接种物寄生了77.2％-89.0％的卵。在温室生产中，与未经处理的土壤相比，接种有15,000衣原体/ g土壤MPc3的番茄植株在根部显示出较少的损害和较少的线虫。在水稻中液体和固体发酵15天后，被MPc3克隆的衣藻的孢子和菌落形成单位（CFU）/ g更大。即使破壁芽孢的发芽百分比小于水稻，破壁玉米双相发酵21天也能使衣原体的产量和CFU / g底物的数量大于水稻中的发酵量。通过延长发酵时间至28天，两种底物中的衣原体孢子浓度均降低。在墨西哥，裂解玉米比大米便宜，可以潜在地与大米生产真菌一样有效。结果证实了这种真菌作为猪笼草的生物防治剂的潜力。

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