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PROTEIN COMPARISON OF SOYBEAN CYST NEMATODE POPULATIONS USING SDS-PAGE

机译：用SDS-PAGE比较大豆囊泡线虫种群的蛋白质

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Donald, P. A., A. N. Patananan, C. A. Cerna, T. Simmons, N. Castro, J. L. Wilgar, and S. C. Goheen. 2008. Protein comparison in Soybean Cyst Nematode Populations using SDS-PAGE. Nematropica 38:137-144. Soybean cyst nematode (SCN, Heterodera glycines) represents one of the most serious threats to predictable soybean yield in the United States. Originally discovered in North Carolina during 1954, intraspecific SCN population variability was soon noted. To reduce SCN crop damage, multiple agricul-ture techniques have been exploited. Of these, resistant varieties and rotation to non host crops have been the most effective in reducing the SCN egg population density. However, no single strategy is effective due to variation in SCN populations, lack of complete resistance, and economics of non host crop production. Although it is well accepted that dissimilarities in virulence phenotypes are not associated with any morphological distinctions, knowledge of biochemical characterization of SCN populations is lacking. Therefore, the protein profiles of eggs from four SCN populations were differentiated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Out of 25 protein bands ranging in molecular weight from 40 to 200 kDa, no differences were observed among any of the populations. These results suggest that eggs from SCN populations differing in virulence phenotypes have similar proteins at the resolution of SDS-PAGE.
机译：唐纳德（Donald），P.A.，A.N。Patananan，C.A.Cerna，T.Simmons，N.Castro，J.L.Wilgar和S.C.Goheen。 2008。使用SDS-PAGE对大豆囊肿线虫种群中的蛋白质进行比较。 Nematropica 38：137-144。大豆囊肿线虫（SCN，异型藻甘氨酸）是美国可预测大豆产量的最严重威胁之一。种内SCN群体变异性最初于1954年在北卡罗来纳州发现，很快就被注意到。为了减少SCN作物的损害，已开发了多种农业技术。其中，抗药性品种和向非寄主作物的轮作在降低SCN卵种群密度方面最有效。但是，由于SCN种群的差异，缺乏完全抗药性以及非寄主农作物生产的经济性，没有一种单一的策略有效。尽管公认毒力表型的差异与任何形态学差异均不相关，但尚缺乏关于SCN种群生物化学表征的知识。因此，使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）区分了四个SCN种群的卵的蛋白质谱。在分子量从40到200 kDa的25条蛋白带中，没有发现任何种群之间的差异。这些结果表明，来自SCN种群的毒力表型不同的卵在SDS-PAGE分辨率下具有相似的蛋白质。

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