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IDENTIFICATION AND GENETIC DIVERSITY OF MELOIDOGYNE CHITWOODI IN POTATO PRODUCTION AREAS OF TURKEY

机译:土耳其马铃薯生产区中甲亚种木聚糖的鉴定和遗传多样性

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Devran, Z., M. Nedim, O. Adem, and E. I. Halil. 2009. Identification and genetic diversity of Meloidogyne chitwoodi in potato production areas of Turkey. Nematropica 39:75-83. Root-knot nematodes (Meloidogyne spp.) were first detected in Turkey during regional nematode surveys of potato fields of Nigde province. Twelve populations of Meloidogyne were collected and identified based on morphological characteristics as well as molecular methods including species-specific primers, confirming that all twelve populations were M. chitwoodi. To estimate genetic relationships among these twelve populations, random amplified polymorphic DNA (RAPD) analysis was done and the results were subjected to UPGM analysis. Analysis of 394 RAPD markers resulted in a similarity coefficient, ranging between 0.64 and 0.81 with a mean similarity of 0.72. The populations Mc3 and Mc4 displayed the greatest genetic similarity (81%). The lowest level of similarity (65%) was observed between Mc9 and Mc7. The large genetic variation among the M. chitwoodi populations indicates that multiple sources of inoculum might have been introduced to the region or that the populations formerly existed in these potato growing areas of Turkey.
机译:Z. Devran,M。Nedim,O。Adem和E.I. Halil。 2009。土耳其马铃薯产区Meloidogyne chitwoodi的鉴定和遗传多样性。 Nematropica 39:75-83。根结线虫(Meloidogyne spp。)是在土耳其对尼格德省马铃薯田进行区域线虫调查时首次发现的。根据形态特征和分子方法(包括物种特异性引物),收集并鉴定了十二种根结线虫种群,确认所有十二个种群均为支原体。为了估计这十二个种群之间的遗传关系,进行了随机扩增多态性DNA(RAPD)分析,并对结果进行了UPGM分析。 394种RAPD标记的分析得出相似系数,范围在0.64和0.81之间,平均相似度为0.72。群体Mc3和Mc4表现出最大的遗传相似性(81%)。在Mc9和Mc7之间观察到最低的相似度(65%)。 Chitwoodi种群之间的巨大遗传变异表明该地区可能已经引入了多种接种物,或者该种群先前存在于土耳其的马铃薯种植区。

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