DEVELOPMENT OF PCR PRIMERS TO IDENTIFY SPECIES OF ROOT-KNOT NEMATODES: MELOIDOGYNE ARENARIA , M. HAPLA , M. INCOGNITA AND M. JAVANICA

K. Dong; R. A. Dean; B. A. Fortnum; S. A. Lewis

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DEVELOPMENT OF PCR PRIMERS TO IDENTIFY SPECIES OF ROOT-KNOT NEMATODES: MELOIDOGYNE ARENARIA , M. HAPLA , M. INCOGNITA AND M. JAVANICA

机译：用于鉴定根结线虫物种的PCR起始物的开发：蛋氨酸肾上腺素，M. HAPLA，M. INCOGNITA和M. JAVANICA

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DNA from twenty-six different single-egg-mass nematode isolates, including seven Meloidogyne arenaria, three M. hapla , eleven M. incognita and five M. javanica , were used to identify species-specific sequence tagged sites. RAPD-PCR was tested to detect species-specific DNA fragments. Nematode isolates of the same species could be grouped unambiguously by most of the polymorphic RAPD patterns. Variations among isolates of each species were also observed, especially within M. arenaria and M. hapla . Potential species-specific DNA fragments from RAPD-PCR were cloned and sequenced. Species- specific PCR primer pairs for M. arenaria , M. hapla , M. incognita and M. javanica were developed.

机译：来自二十六个不同的单卵线虫分离株的DNA，包括七个七倍体球孢子虫，三个黑斑病菌，十一个M. incognita和五个M. javanica，被用来鉴定物种特异性序列标记位点。测试了RAPD-PCR以检测物种特异性DNA片段。相同种类的线虫分离株可以通过大多数多态RAPD模式进行明确分组。还观察到了每个物种的分离株之间的差异，特别是在沙蚕分枝杆菌和哈普拉分枝杆菌内。克隆并测序了来自RAPD-PCR的潜在物种特异性DNA片段。开发了用于沙眼衣原体，黑斑病原菌，隐孢子虫和爪哇分枝杆菌的物种特异性PCR引物对。

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《Nematropica》 |2001年第2期|共10页
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K. Dong; R. A. Dean; B. A. Fortnum; S. A. Lewis;
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1. COMPARATIVE THERMAL-TIME REQUIREMENTS FOR DEVELOPMENT OF MELOIDOGYNE ARENARIA, M. INCOGNITA, AND M. JAVANICA, AT CONSTANT TEMPERATURES [COMPARACIóN DE REQUISITOS TéRMICOS PARA EL DESARROLLO DE MELOIDOGYNE ARENARIA, M. INCOGNITA, AND M. JAVANICA TEMPERATU [J] . M. Dávila-Negrón, D. W. Dickson Nematropica . 2014,第2期

机译：在恒定温度下[[COMPARACION DE REQUISITOSTéRMICOSPARA EL DESARROLLO DE MELOIDOGYNE ARENARIA，M。INCUGNICA，M。INCORATIA]，拟南芥，M。INCOGNITA和M. JAVANICA的比较热时间要求
2. Resistance assessment of grapevine rootstocks used in Chile to the root-knot nematodes Meloidogyne ethiopica, M. hapla, and M. javanica [J] . Erwin Aballay, Oscar Vilches Ciencia e Investigación Agraria . 2016,第3期

机译：智利使用的葡萄砧木对根结线虫Meloidogyne ethiopica，M。hapla和M. javanica的抗性评估
3. Resistance assessment of grapevine rootstocks used in Chile to the root-knot nematodes Meloidogyne ethiopica, M. hapla, and M. javanica [J] . Erwin Aballay, Oscar Vilches Ciencia e Investigación Agraria . 2016,第3期

机译：智利使用的葡萄砧木对根结线虫Meloidogyne ethiopica，M。hapla和M. javanica的抗性评估
4. SUSCEPTIBILITY OF SUGARCANE VARIETIES TO TWO SPECIES OF ROOT-KNOT NEMATODE (MELOIDOGYNE JAVANICA AND M. INCOGNITA), AND IMPLICATIONS FOR CROPS GROWN IN ROTATION WITH SUGARCANE [C] . GRAHAM STIRLING Conference of the Australian Society of Sugar Cane Technologists . 2006

机译：甘蔗品种对两种根结线虫（Meloidogyne Javanica和M.Inmognita）的易感性，以及用甘蔗旋转种植的作物的影响
5. Quantifying Risk for the Northern Root-knot Nematode, Meloidogyne hapla, in Potato in New York State [D] . Gorny, Adrienne Marie 2019

机译：量化纽约州马铃薯北部根结线虫Meloidogyne hapla的风险
6. Susceptibility of Seven Caladium (Caladium × hortulanum) Cultivars to Meloidogyne arenaria M. enterolobii M. floridensis M. incognita and M. javanica [O] . Nancy Kokalis-Burelle, Janete A. Brito, Robert D. Hartman 2017

机译：七个钯（Caladium×hortulanum）品种对南方根结线虫小肠结肠菌弗洛里德氏菌隐孢子虫和爪哇分枝杆菌的敏感性
7. Evaluation of entomopathogenic fungi, Purpureocillium lilacinum TR1 for the control of the Root-knot nematodes (Meloidogyne javanica, M. incognita and M. arenaria) [O] . İlker Kepenekçi, Erçin Oksal 2015

机译：对昆虫致病真菌的评价，紫红素Lilacinum Tr1控制根结线虫（Meloidogyne Javanica，M.Incognita和M.Arenaria）

DEVELOPMENT OF PCR PRIMERS TO IDENTIFY SPECIES OF ROOT-KNOT NEMATODES: MELOIDOGYNE ARENARIA , M. HAPLA , M. INCOGNITA AND M. JAVANICA

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