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首页> 外文期刊>Notulae Botanicae Horti Agrobotanici Cluj-Napoca >Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum)
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Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum)

机译：扭叶大蒜（葱属）和山大蒜（葱属变种）的两个新的蒜氨酸裂解酶（蒜酶）基因

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摘要

Alliinase (Alliin lyase EC 4.4.1.4), a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulfinate, a biologically active compound), pyruvate, and ammonia starting from the specific non-protein sulfur-containing amino acid alliin ((+S)-allyl-L-cysteine sulfoxide). Using newly developed specific primers two new alliinase genes from Allium obliquum and Allium senescens ssp. montanum were amplified and sequenced, as well as their homologs, from Allium fistulosum and Allium schoeonoprasum. The G+C content of the alliinase region ranges between that of other dicot plants and that reported in monocot cereal plants, in all four species. Investigations of gene expression revealed a significantly higher enzyme expression level in bulbs than in leaves in all four taxa. The deduced alliinase sequences displayed a high variability among different species, since the lowest sequence similarity was found to be 55.5% between Allium senescens var. montanum and Allium cepa, while the highest similarity is 77.5%, between Allium senescens var. montanum and Allium fistulosum. Leucine is the most common amino acid in all four alliinases, while cysteine is also more frequent that in other enzymes, suggesting a high stability of the molecules due to the possible disulfide bonds.
机译：蒜氨酸酶（蒜氨酸裂解酶EC 4.4.1.4），一种吡ido醛磷酸盐依赖性裂解酶，代表葱属物种的主要蛋白质成分之一。该酶是同型二聚体糖蛋白，从特定的非蛋白质含硫氨基酸蒜氨酸（（+ S）-烯丙基-L-半胱氨酸）开始催化大蒜素（二烯丙基硫代亚磺酸盐，一种生物活性化合物），丙酮酸和氨的合成。亚砜）。使用新开发的特异性引物，从斜纹葱和葱质葱的两个新的蒜氨酸酶基因。芒草和葱葱中的褐藻及其同系物被扩增和测序。在所有这四个物种中，蒜氨酸酶区域的G + C含量介于其他双子叶植物和单子叶植物中。基因表达的研究表明，在所有四个类群中，鳞茎中的酶表达水平明显高于叶片中的酶表达水平。推导的蒜氨酸酶序列在不同物种之间显示出高变异性，因为发现最低的序列相似性是葱属变种之间的55.5％。 montanum和葱属cepa，最高的相似度是77.5％，在葱属之间。褐煤和葱属瘘。亮氨酸是所有四种蒜氨酸酶中最常见的氨基酸，而半胱氨酸也比其他酶中更常见，这表明由于可能存在的二硫键，分子具有很高的稳定性。

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