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首页> 外文期刊>NPJ vaccines. >Gallid herpesvirus 3 SB-1 strain as a recombinant viral vector for poultry vaccination
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Gallid herpesvirus 3 SB-1 strain as a recombinant viral vector for poultry vaccination

机译：鸡用疱疹病毒3 SB-1株作为家禽疫苗的重组病毒载体

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Live herpesvirus-vectored vaccines are widely used in veterinary medicine to protect against many infectious diseases. In poultry, three strains of herpesvirus vaccines are used against Marek’s disease (MD). However, of these, only the herpesvirus of turkeys (HVT) has been successfully developed and used as a recombinant vaccine vector to induce protection against other avian viral diseases such as infectious bursal disease (IBD), Newcastle disease (ND) or avian influenza (AI). Although effective when administered individually, recombinant HVT vectors have limitations when combined in multivalent vaccines. Thus there is a need for developing additional viral vectors that could be combined with HVT in inducing protection against multiple avian diseases in multivalent vaccines. Gallid herpesvirus 3 (GaHV3) strain SB-1 is widely used by the poultry industry as bivalent vaccine in combination with HVT to exploit synergistic effects against MD. Here, we report the development and application of SB-1 as a vaccine vector to express the VP2 capsid antigen of IBD virus. A VP2 expression cassette was introduced into the SB-1 genome at three intergenic locations (UL3/UL4, UL10/UL11 and UL21/UL22) using recombineering methods on the full-length pSB-1 infectious clone of the virus. We show that the recombinant SB-1 vectors expressing VP2 induced neutralising antibody responses at levels comparable to that of commercial HVT-based VAXXITEKHVT+IBD vaccine. Birds vaccinated with the experimental recombinant SB-1 vaccine were protected against clinical disease after challenge with the very virulent UK661 IBDV isolate, demonstrating its value as an efficient viral vector for developing multivalent vaccines against avian diseases.
机译：活疱疹病毒载体疫苗已广泛用于兽医领域，以预防许多传染病。在家禽中，使用了三种针对马立克氏病（MD）的疱疹病毒疫苗。但是，其中只有火鸡疱疹病毒（HVT）得到成功开发，并用作重组疫苗载体来诱导对其他禽病毒疾病（如传染性法氏囊病（IBD），新城疫（ND）或禽流感（ AI）。尽管重组HVT载体单独给药有效，但与多价疫苗结合使用时仍存在局限性。因此，需要开发可以与HVT结合以诱导针对多价疫苗中的多种禽类疾病的保护的其他病毒载体。禽类疱疹病毒3（GaHV3）菌株SB-1被用作家禽行业的二价疫苗，与HVT结合使用，以发挥针对MD的协同作用。在这里，我们报告SB-1作为疫苗载体表达IBD病毒VP2衣壳抗原的开发和应用。使用重组方法对病毒的全长pSB-1感染性克隆将VP2表达盒引入到三个基因间位置（UL3 / UL4，UL10 / UL11和UL21 / UL22）的SB-1基因组中。我们表明，表达VP2的重组SB-1载体诱导的中和抗体反应水平与基于商业HVT的VAXXITEKHVT + IBD疫苗相当。用非常有毒力的UK661 IBDV分离株攻击后，用实验性重组SB-1疫苗接种的禽类可以预防临床疾病，证明其作为开发抗禽疾病多价疫苗的有效病毒载体的价值。

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