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首页> 外文期刊>Vaccine >Recombinant Gallid herpesvirus 2 with interrupted meq genes confers safe and efficacious protection against virulent field strains
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Recombinant Gallid herpesvirus 2 with interrupted meq genes confers safe and efficacious protection against virulent field strains

机译：具有中断的MEQ基因的重组胆道Herpesvirus 2对毒性田间菌株的安全和有效地保护

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Gallid herpesvirus 2 (GaHV-2) continuously evolves, which reduces the effectiveness of existing vaccines. To construct new GaHV-2 candidate vaccines, LMS, which is a virulent GaHV-2 field strain isolated from diseased chicken flocks in Southwest China in 2007, was modified such that both copies of its meg oncogene were partially deleted. The resulting virus, i.e., rMS Delta meq, was characterized using PCR and sequencing. To evaluate the safety and protective efficacy of rMS Delta meq, specific pathogen-free (SPF) chickens were inoculated with 2000 plaque forming units (pfu) and 20,000 pfu of rMS Delta meq immediately after hatching. All birds grew well during the experimental period, and none of the challenged chickens developed Marek's disease-associated lymphoma. In addition, the rMS Delta meq- and CVI988/Rispens-vaccinated SPF chickens were challenged with 1000 pfu and 5000 pfu of the representative virulent GaHV-2 Md5 strain and 1000 pfu of the variant GaHV-2. strains LCC or LTS. The results showed that the rMS Delta meq strain provided complete protection, which was similar to that provided by the CVI988/Rispens vaccine (protective index (PI) of 95.5) when challenged with a conventional dose of the Md5 strain. However, rMS Delta meq provided a PI of 90.9 when challenged with 5000 pfu of the Md5 strain, which was significantly higher than that provided by the CVI988/Rispens vaccine (54.5). rMS Delta meq provided a PI of 86.4 against LCC, which was equal to that provided by the CVI988/Rispens vaccine (81.8). In addition, rMS Delta meq provided a PI of 100 against LTS, which was significantly higher than that provided by the CVI988/Rispens vaccine (68.2). Altogether, the rMS Delta meq virus provided efficient protection against representative and variant GaHV-2 strains. In conclusion, the rMS Delta meq virus is a safe and effective vaccine candidate for the prevention of Marek's disease and is effective against the Chinese variant GaHV-2 strains. (C) 2017 Published by Elsevier Ltd.
机译：甘草疱疹病毒2（Gahv-2）连续演变，降低了现有疫苗的有效性。为了构建新的GAHV-2候选疫苗，LMS是2007年西南中国西南部患者中分离的毒性GAHV-2野变菌株，使其巨大的肿瘤拷贝分别被部分删除。使用PCR和测序表征得到的病毒，即RMS Delta MEQ。为了评估RMS Delta Meq的安全性和保护效果，在孵化后立即用2000个斑块形成单元（PFU）和20,000pfu的20,000pfu接种特异性病原体（SPF）鸡。所有鸟类在实验期间种植得很好，并且没有挑战性的鸡没有开发MAREK的病情相关淋巴瘤。此外，RMS Delta Meq-and CVI988 / Rispens-accical犬遭受攻击的是1000 pfu和5000 pfu的代表毒力Gahv-2 md5菌株和5000 pfu的变体gahv-2。菌株LCC或LTS。结果表明，RMS DELTA MEQ菌株提供了完全保护，其与CVI988 / RISPENS疫苗（保护指数（PI）为95.5的保护指数（PI）的疫苗，当用常规剂量的MD5菌株攻击时。然而，RMS Delta Meq在用5000 pfu的MD5菌株挑战时提供了90.9的PI，其显着高于CVI988 / RISPENS疫苗（54.5）提供的。 RMS Delta Meq提供了86.4的PI对抗LCC，其等于CVI988 / RISPENS疫苗（81.8）提供的。此外，RMS Delta MEQ提供了100个针对LTS的PI，其显着高于CVI988 / RISPENS疫苗（68.2）提供的PI。完全，RMS Delta MEQ病毒提供了有效的针对代表性和变体GAHV-2菌株的保护。总之，RMS Delta Meq病毒是预防马雷克病的安全有效的疫苗候选者，对中国变异的GAHV-2菌株有效。 （c）2017年由elestvier有限公司出版

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