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The eukaryotic CMG helicase pumpjack and integration into the replisome

机译：真核CMG解旋酶千斤顶并整合到复制体中

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The eukaryotic replisome is?α?multiprotein machine that contains DNA polymerases, sliding clamps, helicase, and primase along with several factors that participate in cell cycle and checkpoint control. The detailed structure of the 11-subunit CMG helicase (Cdc45/Mcm2-7/GINS) has been solved recently by cryoEM single-particle 3D reconstruction and reveals pumpjack motions that imply an unexpected mechanism of DNA translocation. CMG is also the organizing center of the replisome. Recent in vitro reconstitution of leading and lagging strand DNA synthesis has enabled structural analysis of the replisome. By building the replisome in stages from pure proteins, single-particle EM studies have identified the overall architecture of the eukaryotic replisome. Suprisingly leading and lagging strand polymerases bind to opposite faces of the CMG helicase, unlike the long-held view that DNA polymerases are located in back of the helicase to act on the unwound strands.
机译：真核复制体是？α？多蛋白机器，它包含DNA聚合酶，滑动夹，解旋酶和primase以及参与细胞周期和检查点控制的多种因素。 11亚基CMG解旋酶（Cdc45 / Mcm2-7 / GINS）的详细结构最近已通过cryoEM单粒子3D重构解决，并揭示了泵举运动，暗示DNA易位的意外机制。 CMG也是复制体的组织中心。前导链和滞后链DNA合成的最新体外重组使得能够进行复制体的结构分析。通过从纯蛋白逐步构建复制体，单粒子EM研究已经确定了真核复制体的整体结构。令人惊奇的是，前导链和滞后链聚合酶与CMG解旋酶的相对表面结合，这与长期以来一直认为DNA聚合酶位于解旋酶后面以作用于解链链的观点不同。

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