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首页> 外文期刊>Nucleus >Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions
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Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions

机译：果蝇Symplekin动态定位到组蛋白基因体和三细胞交界

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The scaffolding protein Symplekin is part of multiple complexes involved in generating and modifying the 3' end of mRNAs, including cleavage-polyadenylation, histone pre-mRNA processing and cytoplasmic polyadenylation. To study these functions in vivo , we examined the localization of Symplekin during development and generated mutations of the Drosophila Symplekin gene. Mutations in Symplekin that reduce Symplekin protein levels alter the efficiency of both poly A~(+) and histone mRNA 3' end formation resulting in lethality or sterility. Histone mRNA synthesis takes place at the histone locus body (HLB) and requires a complex composed of Symplekin and several polyadenylation factors that associates with the U7 snRNP. Symplekin is present in the HLB in the early embryo when Cyclin E/Cdk2 is active and histone genes are expressed and is absent from the HLB in cells that have exited the cell cycle. During oogenesis, Symplekin is preferentially localized to HLBs during S-phase in endoreduplicating follicle cells when histone mRNA is synthesized. After the completion of endoreplication, Symplekin accumulates in the cytoplasm, in addition to the nucleoplasm, and localizes to tricellular junctions of the follicle cell epithelium. This localization depends on the RNA binding protein ypsilon schachtel. CPSF-73 and a number of mRNAs are localized at this same site, suggesting that Symplekin participates in cytoplasmic polyadenylation at tricellular junctions.
机译：支架蛋白Symplekin是涉及产生和修饰mRNA 3'端的多种复合物的一部分，包括裂解-聚腺苷酸化，组蛋白前-mRNA加工和细胞质聚腺苷酸化。为了在体内研究这些功能，我们研究了Symplekin在发育过程中的定位以及果蝇Symplekin基因的突变。减少Symplekin蛋白水平的Symplekin突变会改变poly A〜（+）和组蛋白mRNA 3'末端形成的效率，从而导致致死性或无菌性。组蛋白mRNA的合成发生在组蛋白基因座体（HLB）处，需要由Symplekin和与U7 snRNP相关的几种聚腺苷酸化因子组成的复合物。当细胞周期蛋白E / Cdk2活跃并且组蛋白基因已经表达并且在退出细胞周期的细胞中不存在时，HLB中的早期胚胎中存在Symplekin。在卵子发生过程中，当合成组蛋白mRNA时，Symplekin在S期中优先定位于HLB，从而消除卵泡细胞。内复制完成后，除核质外，Symplekin还会积聚在细胞质中，并位于滤泡细胞上皮的三细胞连接处。该定位取决于RNA结合蛋白ypsilon schachtel。 CPSF-73和许多mRNA都位于同一位置，这表明Symplekin参与了三细胞交界处的细胞质聚腺苷酸化。

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