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首页> 外文期刊>Nucleus >Coilin is rapidly recruited to UVA-induced DNA lesions and γ-radiation affects localized movement of Cajal bodies
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Coilin is rapidly recruited to UVA-induced DNA lesions and γ-radiation affects localized movement of Cajal bodies

机译：线圈蛋白迅速募集到UVA诱导的DNA损伤中，γ射线影响Cajal体的局部运动

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摘要

Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with γ-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in nucleoli of mouse ES cells.
机译：卡哈尔体是重要的核结构，其中含有优先调节RNA相关代谢的蛋白质。我们研究了未辐射和辐射的人类肿瘤细胞系和胚胎干（ES）细胞中Cajal体的细胞类型特异性核分布和线圈蛋白（Cajal体的蛋白质）的水平。 Cajal体位于不同的核区室中，包括DAPI贫乏区，发色中心附近和核仁附近。每个细胞核的Cajal体数目与细胞周期有关，在G2期发生的数目更高。人ES细胞在核质中含有较高的卷蛋白水平，但在小鼠和人ES细胞的细胞核中也鉴定出了卷蛋白阳性的Cajal体。 Coilin（而非SMN）识别UVA诱导的DNA损伤，该损伤与细胞周期无关。与DNA损伤中GFP-coilin的回收相比，γ射线处理减少了许多细胞类型中Cajal体的局部运动，并且在核质中光漂白后GFP-coilin的荧光恢复非常快。相比之下，核仁定位的线圈蛋白在光漂白后显示出非常缓慢的荧光恢复，这表明蛋白质扩散的速度非常慢，尤其是在小鼠ES细胞的核仁中。

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