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The structure of lamin filaments in somatic cells as revealed by cryo-electron tomography

机译：低温电子断层扫描显示体细胞中层状细纤维的结构

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Metazoan nuclei are equipped with nuclear lamina - a thin layer of intermediate filaments (IFs) mostly built of nuclear lamins facing the inner nuclear membrane (INM). The nuclear lamina serves as an interaction hub for INM-proteins, soluble nuclear factors and DNA. It confers structural and mechanical stability to the nucleus, transduces mechanical forces and biochemical signals across the nuclear envelope (NE) and regulates the organization of chromatin. By using cryo-electron tomography (cryo-ET), we recently provided an unprecedented view into the 3D organization of lamin filaments within the lamina meshwork in mammalian somatic cells. Through implementation of averaging procedures, we resolved the rod and globular Ig-fold domains of lamin filaments. The density maps suggested that they assemble into 3.5?nm thick filaments. Our analysis revealed interesting structural differences between nucleoplasmic and cytoplasmic intermediate filaments, raising the question of which molecular cues define their assembly modes inside the cell.
机译：后生动物的核配备有核薄层-薄层中间丝（IFs），大部分由面对内部核膜（INM）的核薄层构成。核层是INM蛋白，可溶性核因子和DNA的相互作用中心。它赋予核结构和机械稳定性，在整个核包膜（NE）内传递机械力和生化信号，并调节染色质的组织。通过使用低温电子断层扫描（cryo-ET），我们最近提供了前所未有的视角来观察哺乳动物体细胞中层板网状结构中层状细丝的3D组织。通过实施平均程序，我们解决了纤细细丝的杆状和球状Ig折叠域。密度图表明它们组装成3.5纳米厚的细丝。我们的分析揭示了核质和细胞质中间丝之间有趣的结构差异，提出了一个问题，即哪些分子提示定义了它们在细胞内的组装方式。

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