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The structure of lamin filaments in somatic cells as revealed by cryo-electron tomography

机译：冷冻电子断层扫描揭示的体细胞中Lamin长丝的结构

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Metazoan nuclei are equipped with nuclear lamina - a thin layer of intermediate filaments (IFs) mostly built of nuclear lamins facing the inner nuclear membrane (INM). The nuclear lamina serves as an interaction hub for INM-proteins, soluble nuclear factors and DNA. It confers structural and mechanical stability to the nucleus, transduces mechanical forces and biochemical signals across the nuclear envelope (NE) and regulates the organization of chromatin. By using cryo-electron tomography (cryo-ET), we recently provided an unprecedented view into the 3D organization of lamin filaments within the lamina meshwork in mammalian somatic cells. Through implementation of averaging procedures, we resolved the rod and globular Ig-fold domains of lamin filaments. The density maps suggested that they assemble into 3.5nm thick filaments. Our analysis revealed interesting structural differences between nucleoplasmic and cytoplasmic intermediate filaments, raising the question of which molecular cues define their assembly modes inside the cell.
机译：美容核核配备有核薄膜 - 薄层中间细丝（IFS），主要由面向内核膜（INM）的核叠层构成。核薄片用作INM-蛋白的相互作用枢纽，可溶性核因子和DNA。它赋予核结构和机械稳定性，在核包膜（NE）上转换机械力和生化信号，并调节染色质的组织。通过使用Cryo-Electro-Electron Intraphy（Cryo-et），我们最近向哺乳动物体细胞的Lamina Meshwork内的Lamin长丝的3D组织提供了前所未有的视图。通过实施平均程序，我们解决了Lamin长丝的杆和球状Ig折叠结构域。密度图表明它们组装成3.5nm厚的长丝。我们的分析揭示了骨髓和细胞质中间细丝之间有趣的结构差异，提高了哪些分子提示在细胞内定义其组装模式的问题。

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