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Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana

机译：Nup82与Nup136在水杨酸依赖性拟南芥防御反应中具有多余功能

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The nuclear pore complex (NPC) comprises more than 30 nucleoporins (Nups). NPC mediates macromolecular trafficking between the nucleoplasm and the cytoplasm, but specific roles of individual Nups are poorly understood in higher plants. Here, we show that the novel nucleoporin unique to angiosperm plants (designated as Nup82) functions in a salicylic acid-dependent defense in a redundant manner with Nup136, which is a component of the nuclear basket in the NPC. Arabidopsis thaliana Nup82 had a similar amino acid sequence to the N-terminal half of Nup136 and a Nup82-GFP fusion was localized on the nuclear envelope. Immunoprecipitation and bimolecular fluorescence complementation analyses revealed that Nup82 interacts with the NPC components Nup136 and RAE1. The double knockout mutant nup82 nup136 showed severe growth defects, while the single knockout mutant nup82 did not, suggesting that Nup82 functions redundantly with Nup136. nup82 nup136 impaired benzothiadiazole (an analog of salicylic acid)-induced resistance to the virulent bacteria Pseudomonas syringae pv. tomato DC3000. Furthermore, transcriptome analysis of nup82 nup136 indicates that deficiency of Nup82 and Nup136 causes noticeable downregulation of immune-related genes. These results suggest that Nup82 and Nup136 are redundantly involved in transcriptional regulation of salicylic acid-responsive genes through nuclear transport of signaling molecules.
机译：核孔复合物（NPC）包含30多个核孔蛋白（Nups）。 NPC介导核质和细胞质之间的大分子运输，但在高等植物中对单个小睡的特定作用知之甚少。在这里，我们显示了被子植物唯一的新型核孔蛋白（命名为Nup82）在水杨酸依赖性防御中以多余的方式与Nup136结合发挥作用，Nup136是NPC中核篮子的组成部分。拟南芥Nup82具有与Nup136的N末端一半相似的氨基酸序列，并且Nup82-GFP融合物位于核被膜上。免疫沉淀和双分子荧光互补分析显示Nup82与NPC组件Nup136和RAE1相互作用。双敲除突变体nup82 nup136显示出严重的生长缺陷，而单敲除突变体nup82没有，这表明Nup82与Nup136冗余地起作用。 nup82 nup136损害了苯并噻二唑（水杨酸的类似物）诱导的对强力细菌丁香假单胞菌pv的抗性。 番茄DC3000。此外，对nup82 nup136的转录组分析表明，Nup82和Nup136的缺乏会引起免疫相关基因的显着下调。这些结果表明，Nup82和Nup136通过信号分子的核转运过多地参与了水杨酸反应基因的转录调控。

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