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首页> 外文期刊>Nucleus >Molecular characterization of Xenopus lamin LIV reveals differences in the lamin composition of sperms in amphibians and mammals
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Molecular characterization of Xenopus lamin LIV reveals differences in the lamin composition of sperms in amphibians and mammals

机译：爪蟾腔隙蛋白LIV的分子表征揭示了两栖动物和哺乳动物精子腔隙蛋白组成的差异

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Lamins are nuclear intermediate filament proteins. They are involved in most nuclear activities and are essential for retaining the mechano-elastic properties of the nucleus. Somatic cells of vertebrates express lamins A, B1, and B2 while lamin LIII, a major component of the amphibian oocyte lamina is absent in mammals. The organization of the lamina of germ cells differs significantly from that of somatic cells. Mammalian spermatogenic cells express two short lamins, C2 and B3, that are splice isoforms of lamin A and B2, respectively. Here we identify the previously described Xenopus lamin LIV as splice variant of the lamin LIII gene. LIV contains 40 extra residues in coil 2A of the rod domain, which results in altered assembly properties. Xenopus lamin LIV and mammalian B3 assemble into short structures rather than into long IF-like filaments. Expression of lamin LIV is restricted to male germ cells suggesting that it might be the functional equivalent of mammalian lamin B3. We provide evidence that lamins C2 and B3 are restricted to the mammalian lineage and describe the lamin composition of Xenopus sperm. Our results show that the evolution of germ cell-specific lamins followed separate and distinctly different paths in amphibians and mammals.
机译：核纤层蛋白是核中间丝蛋白。它们参与大多数核活动，对于保留核的机械弹性特性至关重要。脊椎动物的体细胞表达lamin A，B1和B2，而lamin LIII（两栖卵母细胞层的主要成分）在哺乳动物中不存在。生殖细胞的层状组织与体细胞的层状组织显着不同。哺乳动物生精细胞表达两个短纤层蛋白C2和B3，它们分别是层粘蛋白A和B2的剪接同工型。在这里，我们确定先前描述的非洲爪蟾lamin LIV作为lamin LIII基因的剪接变体。 LIV在棒结构域的线圈2A中包含40个额外的残基，这导致装配特性发生了变化。非洲爪蟾的lamin LIV和哺乳动物B3组装成短结构，而不是长的IF样细丝。 Lamin LIV的表达仅限于雄性生殖细胞，表明它可能与哺乳动物Lamin B3的功能相当。我们提供了lamins C2和B3仅限于哺乳动物谱系的证据，并描述了非洲爪蟾精子的lamin组成。我们的结果表明，在两栖动物和哺乳动物中，生殖细胞特异性lamin的进化遵循不同且截然不同的路径。

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