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首页> 外文期刊>Nutrition Research and Practice >Effects of parental folate deficiency on the folate content, global DNA methylation, and expressions of FRα, IGF-2 and IGF-1R in the postnatal rat liver
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Effects of parental folate deficiency on the folate content, global DNA methylation, and expressions of FRα, IGF-2 and IGF-1R in the postnatal rat liver

机译：父母叶酸缺乏对出生后大鼠肝脏叶酸含量，整体DNA甲基化以及FRα，IGF-2和IGF-1R表达的影响

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>We examined the effect of parental folate deficiency on the folate content, global DNA methylation, folate receptor-alpha (FRα), insulin-like-growth factor-2 (IGF-2) and -1 receptor (IGF-1R) in the liver and plasma homocysteine in the postnatal rat. Male and female rats were randomly fed a folic acid-deficient (paternal folate-deficient, PD and maternal folate-deficient, MD), or folic acid-supplemented diet (paternal folate-supplemented, PS and maternal-folate-supplemented, MS) for four weeks. They were mated and grouped accordingly: PSxMS, PSxMD, PDxMS, and PDxMD. Pups were killed on day 21 of lactation. The hepatic folate content was markedly reduced in the PDxMD and PSxMD and PDxMS as compared with the PSxMS group. The hepatic global DNA methylation was decreased in the PDxMS and PSxMD groups as much as in the PDxMD group, and all the three groups were significantly lower as compared to the PSxMS group. There were no significant differences in the hepatic FRα, IGF-2 and IGF-1R expressions among the groups. Positive correlations were found between the hepatic folate content and global DNA methylation and protein expressions of FRα, IGF-2 and IGF-1R, whereas an inverse correlation was found between hepatic folate content and plasma homocysteine level in the 3-week-old rat pup. The results of this study show that both paternal and maternal folate deficiency at mating can influence the folate content and global DNA methylation in the postnatal rat liver.
机译：>我们检查了父母叶酸缺乏对叶酸含量，总体DNA甲基化，叶酸受体α（FRα），胰岛素样生长因子2（IGF-2）和-1受体（IGF-1R）的影响在产后大鼠的肝脏和血浆同型半胱氨酸中。随机给雄性和雌性大鼠喂食叶酸不足（PD）和母体叶酸缺乏（MD）或叶酸补充饮食（PS，PS和母体叶酸补充，MS）四个星期。将它们配对并分组：PSxMS，PSxMD，PDxMS和PDxMD。在哺乳的第21天，幼仔被杀死。与PSxMS组相比，PDxMD和PSxMD和PDxMS中的肝叶酸含量明显降低。与PDxMD组相比，PDxMS和PSxMD组的肝脏总体DNA甲基化水平下降，并且与PSxMS组相比，三组的肝总DNA甲基化水平均显着降低。各组之间肝FRα，IGF-2和IGF-1R表达无明显差异。在3周龄的幼仔中，肝叶酸含量与FRα，IGF-2和IGF-1R的整体DNA甲基化和蛋白质表达之间呈正相关，而肝叶酸含量与血浆同型半胱氨酸水平呈负相关。 。这项研究的结果表明，交配时父亲和母亲的叶酸缺乏都会影响出生后大鼠肝脏中的叶酸含量和总体DNA甲基化。

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