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首页> 外文期刊>Nutrients >(?)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
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(?)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway

机译：（？）-Epigallocatechin-3-Gallate通过抑制Wnt /β-Catenin途径抑制大肠癌干细胞

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The beneficial effects of tea consumption on cancer prevention have been generally reported, while (?)-Epigallocatechin-3-gallate (EGCG) is the major active component from green tea. Cancer stem cells (CSCs) play a crucial role in the process of cancer development. Targeting CSCs may be an effective way for cancer intervention. However, the effects of EGCG on colorectal CSCs and the underlying mechanisms remain unclear. Spheroid formation assay was used to enrich colorectal CSCs from colorectal cancer cell lines. Immunoblotting analysis and quantitative real-time polymerase chain reaction were used to measure the alterations of critical molecules expression. Immunofluorescence staining analysis was also used to determine the expression of CD133. We revealed that EGCG inhibited the spheroid formation capability of colorectal cancer cells as well as the expression of colorectal CSC markers, along with suppression of cell proliferation and induction of apoptosis. Moreover, we illustrated that EGCG downregulated the activation of Wnt/β-catenin pathway, while upregulation of Wnt/β-catenin diminished the inhibitory effects of EGCG on colorectal CSCs. Taken together, this study suggested that EGCG could be an effective natural compound targeting colorectal CSCs through suppression of Wnt/β-catenin pathway, and thus may be a promising agent for colorectal cancer intervention.
机译：茶饮对预防癌症的有益作用已得到普遍报道，而（β）-表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶的主要活性成分。癌症干细胞（CSC）在癌症发展过程中起着至关重要的作用。靶向CSCs可能是癌症干预的有效方法。但是，EGCG对结直肠CSC的作用及其潜在机制尚不清楚。球体形成测定法用于从结肠直肠癌细胞系中富集结肠直肠CSC。免疫印迹分析和定量实时聚合酶链反应用于衡量关键分子表达的变化。免疫荧光染色分析还用于确定CD133的表达。我们发现，EGCG抑制大肠癌细胞的球状形成能力以及大肠CSC标记的表达，以及抑制细胞增殖和诱导细胞凋亡。此外，我们表明，EGCG下调了Wnt /β-catenin途径的激活，而Wnt /β-catenin的上调则减少了EGCG对结直肠CSC的抑制作用。两者合计，这项研究表明，EGCG可能是通过抑制Wnt /β-catenin途径靶向大肠CSCs的有效天然化合物，因此可能是大肠癌干预的有希望的药物。

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