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首页> 外文期刊>Drug Design, Development and Therapy >Thymol Isolated from Thymus vulgaris L. Inhibits Colorectal Cancer Cell Growth and Metastasis by Suppressing the Wnt/β-Catenin Pathway
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Thymol Isolated from Thymus vulgaris L. Inhibits Colorectal Cancer Cell Growth and Metastasis by Suppressing the Wnt/β-Catenin Pathway

机译：从胸腺寻常的L.分离的胸腺抑制通过抑制Wnt /β-catenin途径来抑制结肠直肠癌细胞生长和转移

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Purpose: Colorectal cancer (CRC) is one of the most commonly occurring cancers and is associated with high morbidity and mortality. Nevertheless, there is currently no safe and effective treatment for this condition. Thymol is a phenolic compound that is recognized as safe for use in food as well as medical and cosmetic fields. Increasing evidence has indicated that thymol exerts prominent antitumor effects in a variety of cancers, including CRC. However, how thymol elicits these effects on CRC and the associated underlying mechanisms remains unclear. Methods: HCT116 and Lovo cells were treated with different concentrations of thymol. Cell Counting Kit-8 (CCK-8) and transwell migration and invasion assays were used to evaluate cell proliferation, migration, and invasion, respectively. Cell apoptosis and cell cycle distribution were measured by flow cytometry. RT-qPCR, Western blot, and immunohistochemistry were used to detect the expression of related genes and their protein products. Results: In this study, we tested the antitumor activity of thymol extracted from a Chinese medicinal herb, Thymus vulgaris L. We show that thymol treatment in vitro inhibited cell proliferation and induced apoptosis and cell cycle arrest in CRC. Furthermore, in vivo treatment with 75 and 150 mg/kg thymol led to a significant decrease in tumor volume. Thymol administration induced CRC cell apoptosis through activation of the BAX/Bcl-2 signaling pathway. In addition, thymol suppressed CRC cell epithelial–mesenchymal transition (EMT), invasion, and metastasis via inhibiting the activation of the Wnt/β-catenin pathway, both in vitro and in vivo. Conclusion: Thymol may prevent CRC progression through inhibition of the Wnt/β-catenin signaling pathway, highlighting its potential as a novel therapeutic option for the treatment of CRC.
机译：目的：结肠直肠癌（CRC）是最常见的癌症之一，与高发病率和死亡率有关。尽管如此，目前没有安全有效的治疗这种情况。胸腺醇是一种酚类化合物，其被认为是在食品以及医学和化妆品领域使用的安全。越来越多的证据表明胸腺醇在包括CRC中的各种癌症中施加着突出的抗肿瘤作用。然而，Thymol如何引发这些影响CRC，相关的潜在机制仍然不清楚。方法：用不同浓度的百里酚处理HCT116和Lovo细胞。使用细胞计数试剂盒-8（CCK-8）和Transwell迁移和侵袭测定分别评估细胞增殖，迁移和侵袭。通过流式细胞术测量细胞凋亡和细胞周期分布。 RT-QPCR，Western印迹和免疫组织化学用于检测相关基因及其蛋白质产品的表达。结果：在本研究中，我们测试了从中药草药中提取的胸腺酚的抗肿瘤活性，胸腺vulgaris L.我们表明体外抑制细胞增殖和诱导CRC诱导细胞凋亡和细胞循环捕获的抑制细胞治疗。此外，体内用75和150mg / kg百里酚的处理导致肿瘤体积显着降低。胸腺溶剂给药通过激活Bax / Bcl-2信号通路诱导CRC细胞凋亡。此外，谷植物抑制CRC细胞上皮 - 间充质转换（EMT），侵袭和转移，抑制体外和体内WNT /β-连环蛋白途径的活化。结论：胸腺醇可以通过抑制Wnt /β-catenin信号传导途径来防止CRC进展，突出其作为用于治疗CRC的新型治疗选择的潜力。

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