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首页> 外文期刊>Nitrogen >Biosensor-Mediated In Situ Imaging Defines the Availability Period of Assimilatory Glutamine in Maize Seedling Leaves Following Nitrogen Fertilization
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Biosensor-Mediated In Situ Imaging Defines the Availability Period of Assimilatory Glutamine in Maize Seedling Leaves Following Nitrogen Fertilization

机译：生物传感器介导的原位成像定义了氮肥后玉米幼苗叶片中同化谷氨酰胺的有效期

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The amino acid glutamine (Gln) is an important assimilatory intermediate between root-derived inorganic nitrogen (N) (i.e., ammonium) and downstream macromolecules, and is a central regulator in plant N physiology. The timing of Gln accumulation after N uptake by roots has been well characterized. However, the duration of availability of accumulated Gln at a sink tissue has not been well defined. Measuring Gln availability would require temporal measurements of both Gln accumulation and its reciprocal depletion. Furthermore, as Gln varies spatially within a tissue, whole-organ in situ visualization would be valuable. Here, the accumulation and subsequent disappearance of Gln in maize seedling leaves (Zea mays L.) was imaged in situ throughout the 48 h after N application to roots of N-deprived plants. Free Gln was imaged by placing leaves onto agar embedded with bacterial biosensor cells (GlnLux) that emit luminescence in the presence of leaf-derived Gln. Seedling leaves 1, 2, and 3 were imaged simultaneously to measure Gln availability across tissues that potentially vary in N sink strength. The results show that following root N fertilization, free Gln accumulates and then disappears with an availability period of up to 24 h following peak accumulation. The availability period of Gln was similar in all seedling leaves, but the amount of accumulation was leaf specific. As Gln is not only a metabolic intermediate, but also a signaling molecule, the potential importance of regulating its temporal availability within plant tissues is discussed.
机译：氨基酸谷氨酰胺（Gln）是根源性无机氮（N）（铵）和下游大分子之间的重要同化中间物，是植物N生理学的主要调节剂。根吸收氮后Gln积累的时间已被很好地表征。然而，在汇组织上积累的Gln的可用性持续时间尚未明确。测量Gln的可用性将需要同时测量Gln的积累及其相互消耗。此外，由于Gln在组织内的空间变化，整个器官的原位可视化将很有价值。在这里，在向缺氮植物的根部施氮48小时后，玉米幼苗叶片（Zea mays L.）中Gln的积累和随后的消失被原位成像。通过将叶子放在装有细菌生物传感器细胞（GlnLux）的琼脂上对游离Gln进行成像，该细菌生物传感器细胞在存在叶衍生Gln的情况下会发光。同时对幼苗叶片1、2和3进行成像，以测量跨N汇强度可能变化的组织中的Gln利用率。结果表明，在施氮后，游离Gln积累，然后在峰值积累后长达24 h的有效期内消失。 Gln的有效期在所有幼苗叶片中都相似，但是积累量是叶片特异性的。由于Gln不仅是代谢中间体，而且还是信号分子，因此讨论了调节其在植物组织中的时间可用性的潜在重要性。

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