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首页> 外文期刊>Leukemia >A flow cytometric method to detect apoptosis-related protein expression in minimal residual disease in acute myeloid leukemia|[ast]|
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A flow cytometric method to detect apoptosis-related protein expression in minimal residual disease in acute myeloid leukemia|[ast]|

机译：流式细胞术检测急性髓样白血病微弱残留病中凋亡相关蛋白的表达

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Minimal residual disease (MRD) cells are thought to be responsible for the persistence and relapse of acute myeloid leukemia (AML). Flow cytometric MRD detection by the establishment of a leukemia-associated phenotype (LAP) at diagnosis can be used in 80% of AML patients, allowing detection and functional characterization of MRD in follow-up bone marrow. One of the mechanisms contributing to inefficient chemotherapy is apoptosis resistance. Measuring apoptosis parameters in MRD cells will help to unravel the importance of apoptosis resistance in AML. We therefore developed a four-color flow cytometry method that enables establishment of apoptosis-related protein expression such as Bcl-2, Bcl-xL, Mcl-1 and Bax at diagnosis and in MRD. Firstly, validation of this assay using Western blot analysis in five leukemia cell lines showed a significant correlation (R=0.70: PP= 0.57, follow-up: P= 0.43). The flow cytometric protocol thus enables analysis of apoptosis-related proteins at different stages of the disease, which will lead to a better understanding of the role of apoptosis resistance in the emergence of MRD in AML.
机译：最小残留疾病（MRD）细胞被认为是急性髓细胞性白血病（AML）持续和复发的原因。通过在诊断时建立与白血病相关的表型（LAP）建立流式细胞仪检测MRD可以用于80％的AML患者，从而可以在随访骨髓中检测MRD和对其功能进行表征。导致化疗无效的机制之一是细胞凋亡抗性。测量MRD细胞的凋亡参数将有助于阐明AML中凋亡抗性的重要性。因此，我们开发了一种四色流式细胞术，可以在诊断和MRD中建立凋亡相关蛋白的表达，例如Bcl-2，Bcl-xL，Mcl-1和Bax。首先，在五个白血病细胞系中使用蛋白质印迹分析对该试验进行验证显示出显着的相关性（R = 0.70：PP = 0.57，随访：P = 0.43）。因此，流式细胞术方案能够分析疾病不同阶段的凋亡相关蛋白，这将使人们更好地了解AML中MRD出现中凋亡抗性的作用。

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