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首页> 外文期刊>Latin american journal of aquatic research >Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837)
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Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837)

机译：卵表面糖缀合物在虾类Rhynchocinetes typus的受精中的作用（Milne-Edwards，1837）

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摘要

During first gamete interaction, oligosaccharides on the glycoproteins present in oocyte envelopes of the rock shrimp, Rhynchocinetes typus, play an important role in spermatozoon recognition prior to the onset of penetration. These oligosaccharides have terminal monosaccharides that may be recognized by means of specific lectins. A variety of marine species have monosaccharides on their oocyte envelopes, allowing them to interact with the spermatozoa. The number and location of the monosaccharides varies during oocyte maturation. However, this phenomenon has not been studied in shrimp. The present study evaluates the presence and location of monosaccharides on oocyte envelopes during maturation using seven specific lectins conjugated to FITC (GNL, LCA, Con-A BS-1, LTA, GLS-II,DBA); these recognize mannose (α 1-3) mannose, α-mannose, α-glucose, glucose, galactose, fucose, N-acetyl-glucosamine, and N-acetyl-galactosamine, respectively. Participation of these carbohydrates in the fertilization process was determined through the insemination of fresh oocytes with spermatozoa previously incubated with the different monosaccharides that block the active sites of the spermatozoa, inhibiting fertilization. The results showed the presence of N-acetyl-glucosamine and mannose on the oocyte envelopes, indicating that these sugars could be the receptors during the first gamete interaction. The results also suggested that glucose could be a complementary receptor to N-acetyl-glucosamine and mannose since, despite the low concentration of glucose on the envelope, this sugar generated a high degree of fertilization inhibition.
机译：在第一次配子相互作用期间，存在于岩虾卵型Rhynchocinetes typus卵母细胞包膜中的糖蛋白上的寡糖在渗透之前对精子的识别中起重要作用。这些寡糖具有末端单糖，其可以通过特定的凝集素识别。各种各样的海洋物种在卵母细胞包膜上都有单糖，从而使其与精子相互作用。在卵母细胞成熟过程中，单糖的数量和位置有所不同。但是，尚未在虾中研究这种现象。本研究使用七个与FITC偶联的特定凝集素（GNL，LCA，Con-A BS-1，LTA，GLS-II，DBA）评估成熟过程中卵母细胞包膜上单糖的存在和位置;它们分别识别甘露糖（α1-3）甘露糖，α-甘露糖，α-葡萄糖，葡萄糖，半乳糖，岩藻糖，N-乙酰基葡萄糖胺和N-乙酰基半乳糖胺。这些碳水化合物参与受精过程的方法是，用新鲜的卵母细胞用精子进行授精，这些精子事先与不同的单糖一起孵育，这些单糖会阻断精子的活性部位，从而抑制受精。结果表明在卵母细胞包膜上存在N-乙酰基-氨基葡萄糖和甘露糖，表明这些糖可能是第一次配子相互作用期间的受体。该结果还表明，葡萄糖可以是N-乙酰基-葡萄糖胺和甘露糖的互补受体，因为尽管包膜上的葡萄糖浓度较低，但这种糖仍能高度抑制受精。

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