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首页> 外文期刊>Reproductive Biology and Endocrinology >RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients
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RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients

机译：卵丘细胞中RUNX2，GPX3和PTX3基因表达谱是反射性卵母细胞/胚胎能力，是PCOS患者胚胎发育能力的潜在可靠预测指标

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Background Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The developmental competence of oocytes and embryos in PCOS patients is reduced to a certain extent (comparing to non-PCOS patients, the high quality embryo rate was decreased by 16% from the data of our centre) during the in vitro fertilization (IVF) process. Cross-talk between the oocyte and cumulus cells is critical for oocyte maturation and embryo competence. In this study, we have evaluated the transcription of specific genes in cumulus cells harvested from pre-ovulatory follicles of PCOS patients before IVF, according to individual oocyte nuclear maturity and developmental competence. Seven genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, and ITGB5) were targeted from our previous cDNA microarray data which isolated genes related to oocyte nuclear maturation in PCOS patients. Two additional genes which had been found to be associated with oocyte maturation or embryo quality in non-PCOS patients (GPX3 and PTX3) were also studied. Methods The mRNA expression levels of cumulus cells were detected by qRT- PCR. Results Consistent with our previous cDNA microarray data, with the exception of GPX3 and PTX3, the selected 7 genes were related to oocyte nuclear maturation in PCOS patients. Noticeably, the expression level of RUNX2 was lower in cumulus cells derived from oocytes that could develop into blastocysts than the level of expression from oocytes that could not. The PTX3 expression level was significantly lower in cumulus cells from oocytes with two normal pronuclei than that from oocytes that formed >2 pronuclei (MPN) after fertilization. GPX3 mRNA levels were decreased in cumulus cells isolated from oocytes that developed into blastocysts with high potential development competence. Conclusions Several cumulus cell genes were associated with oocyte maturation, fertilization and embryo quality in PCOS patients. RUNX2 and GPX3 are candidate genetic markers in the monitoring of embryo quality for PCOS patients, whereas PTX3 mainly played a role in fertilization process. Together with morphological evaluation, cumulus cells genes may serve as biomarkers of oocyte and embryo selection during the IVF process for PCOS patients and may advance our understanding of PCOS.
机译：背景技术多囊卵巢综合征（PCOS）是女性常见的内分泌和代谢疾病。在体外受精（IVF）过程中，PCOS患者的卵母细胞和胚胎的发育能力有所降低（与非PCOS患者相比，高质量的胚胎率比我们中心的数据降低了16％） 。卵母细胞与卵丘细胞之间的串扰对卵母细胞成熟和胚胎能力至关重要。在这项研究中，我们根据卵母细胞的核成熟度和发育能力，评估了IVF前PCOS患者排卵前卵泡收集的卵丘细胞中特定基因的转录。从我们先前的cDNA微阵列数据中，靶向了七个基因（RUNX2，PSAT1，ADAMTS9，CXCL1，CXCL2，CXCL3和ITGB5），这些数据分离了与PCOS患者卵母细胞核成熟相关的基因。还研究了另外两个与非PCOS患者的卵母细胞成熟或胚胎质量有关的基因（GPX3和PTX3）。方法采用qRT-PCR技术检测卵丘细胞的mRNA表达水平。结果与我们以前的cDNA微阵列数据一致，除GPX3和PTX3外，所选的7个基因与PCOS患者的卵母细胞核成熟有关。值得注意的是，RUNX2在可发育为胚泡的卵母细胞的卵丘细胞中的表达水平低于不能卵母细胞的卵丘细胞的表达水平。具有两个正常原核的卵母细胞的卵丘细胞中PTX3表达水平显着低于受精后形成> 2原核（MPN）的卵母细胞。从卵母细胞分离的卵丘细胞中GPX3 mRNA水平下降，卵母细胞发育为具有高潜在发展能力的胚泡。结论PCOS患者的几个卵丘细胞基因与卵母细胞的成熟，受精和胚胎质量有关。 RUNX2和GPX3是监测PCOS患者胚胎质量的候选遗传标记，而PTX3主要在受精过程中发挥作用。结合形态学评估，积石细胞基因可能在PCOS患者的IVF过程中充当卵母细胞和胚胎选择的生物标记，并可能增进我们对PCOS的理解。

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