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首页> 外文期刊>Reproductive Biology and Endocrinology >Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants
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Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants

机译：微囊泡介导的可溶性LH / hCG受体（LHCGR）从转染的细胞和胎盘外植体中释放

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摘要

Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. Secretion was minimal up to 72h but by 96h 24-37% of the LHCGR had been released into the culture medium. The secreted proteins were folded and sensitive to glycosidases suggesting N-linked glycosylation. Secretion was independent of recombinant size and was mediated via structurally defined membrane vesicles (50-150nm). Similarly cultured human early pregnancy placental explants also released LHCGR via microvesicles. These studies provide the first experimental evidence of the possible mechanistic basis of the secretion of LHCGR.
机译：胎盘hCG和垂体LH通过共同受体（LHCGR）在靶组织中转导信号。我们证明重组LHCGR蛋白质，包括激素结合域是从转染的细胞中分泌出来的，而天然LHCGR也是从人胎盘外植体中分泌出来的。在悬浮培养中，在中国仓鼠卵巢细胞中表达了代表N末端细胞外结构域不同长度的LHCGR重组蛋白。直到72小时分泌最少，但是到96小时，LHCGR的24-37％已经释放到培养基中。分泌的蛋白质被折叠并且对糖苷酶敏感，表明N-连接的糖基化。分泌与重组体大小无关，并通过结构确定的膜囊泡（50-150nm）介导。同样培养的人类早孕胎盘外植体也通过微泡释放LHCGR。这些研究提供了LHCGR分泌的可能机制基础的第一个实验证据。

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