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首页> 外文期刊>Research Journal of Pharmaceutical Sciences >Effect of Explants and Plant Growth Regulators on Callus Induction in Ricinus communis L
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Effect of Explants and Plant Growth Regulators on Callus Induction in Ricinus communis L

机译：外植体和植物生长调节剂对蓖麻愈伤组织诱导的影响

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The present research work aimed to shedding light on the effect of various plant growth regulator namely 2,4 dichlorophenoxy acetic acid (2,4-D) and -Naphthalene Acetic Acid (NAA) on callus induction of ricinus commun. Callus culture were initiated from cotyledonary leaf and root segments explants from in vitro Ricinus communis L seedling . After four day of inoculation callusing was observed from cotyledonary leaf segment explants, after about one week from root segment explant. Auxins 2,4-D and NAA effecting in callus agitationbut 2.5 mg/L 2,4-D proved to be more effected for induction of callus in R. communis L. Individual treatment of NAA reveal low effectiveness for callus induction. Among all treatment media comprising 2,4 D (2.5 mg/l) showed superior response of callus degree (4 .0-0.0) with cotyledonary leaf segment explant and at this concentration, maximum amount of callus was observed whereas, root segment explants showed (2.7-0.19 ) degree of callus response in the same concentration . The callus from cotyledonary leaf segment explants was Light brown colour with compact nature in media fortified with 2,4 D while in media fortified with NAA callus was Light green colour with Friable nature. The callus from root segment explants was creamy to yellow colour with compact nature with the both tested auxin.
机译：本研究工作旨在阐明各种植物生长调节剂，即2,4-二氯苯氧基乙酸（2,4-D）和-萘乙酸（NAA）对蓖麻的愈伤组织诱导的影响。从离体蓖麻幼苗的子叶叶片和根段外植体开始愈伤组织培养。接种四天后，观察到子叶叶节外植体的愈伤组织，离根节外植体约一周后出现using愈。生长素2,4-D和NAA会影响愈伤组织的搅动，但是2.5 mg / L 2,4-D被证明对R. communis L.愈伤组织的诱导作用更大。NAA的单独处理显示出较低的愈伤组织诱导效果。在包含2,4 D（2.5 mg / l）的所有处理培养基中，对子叶叶节外植体的愈伤组织度（4 .0-0.0）表现出优异的响应，在此浓度下，观察到最大的愈伤组织，而根节外植体显示在相同浓度下，愈伤组织反应的程度为（2.7-0.19）。子叶叶部分外植体的愈伤组织为浅褐色，在以2,4 D强化的培养基中呈致密性，而在以NAA强化的培养基中，为浅绿色，呈脆性。根部外植体的愈伤组织呈奶油色至黄色，具有两种测试生长素的致密性质。

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