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首页> 外文期刊>Research Journal of Medical Sciences >Phenotypic and Genotypic Characterization of Antimicrobial Resistance among Diarrheagenic Escherichia coli
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Phenotypic and Genotypic Characterization of Antimicrobial Resistance among Diarrheagenic Escherichia coli

机译：腹泻性大肠杆菌之间抗菌素耐药性的表型和基因型特征

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Diarrhea caused by multidrug-resistant bacteria is an important public health problem among children in developing countries. Escherichia coli is an important cause of disease in animals and humans worldwide. Twenty five E. coli isolates with rate 61.2% among human and environments were tested for susceptibility to 10 antimicrobial agents by disk diffusion method. Resistant isolates were screened by molecular methods for resistance genes, TetA, TetB, strepA, MarI and MarII. Molecular result showed that all isolates harbored resistance gene for the TetA, TetB, strepA, MarI, and MarII even though the genotypic test showed sensitive to the drugs. E. coli isolates exhibit a wide repertoire of genetic elements to sustain antimicrobial pressure. The results of this study using pheno-genotypic techniques highlight the distribution of E. coli among human, animal, aquatic ecosystems and the potential public health threat of E. coli originating from municipal wastewater sources.
机译：由多药耐药菌引起的腹泻是发展中国家儿童中的重要公共卫生问题。大肠杆菌是全世界动物和人类疾病的重要原因。通过圆盘扩散法测试了25株在人类和环境中分离率为61.2％的大肠杆菌，对10种抗菌剂的敏感性。通过分子方法针对抗性基因TetA，TetB，strepA，MarI和MarII筛选抗性分离株。分子结果显示，即使基因型测试显示对药物敏感，所有分离株均具有针对TetA，TetB，strepA，MarI和MarII的抗性基因。大肠杆菌分离物显示出广泛的遗传成分以维持抗微生物压力。使用表型基因型技术进行的这项研究的结果突出了大肠杆菌在人，动物，水生生态系统中的分布以及源自城市废水源的大肠杆菌的潜在公共卫生威胁。

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